PMID- 20237096
OWN - NLM
STAT- MEDLINE
DCOM- 20100806
LR  - 20181113
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 48
IP  - 5
DP  - 2010 May
TI  - Phages harboring specific peptides that recognize the N protein of the porcine 
      reproductive and respiratory syndrome virus distinguish the virus from other 
      viruses.
PG  - 1875-81
LID - 10.1128/JCM.01707-09 [doi]
AB  - The aim of the current study was to develop a novel diagnostic test for detecting 
      porcine reproductive and respiratory syndrome virus (PRRSV) using phage display 
      technology. The N gene of PRRSV isolate HH08 was cloned following reverse 
      transcription-PCR. Sequence comparison indicated that the N gene shared 96.4% 
      homology to that of North American PRRSV (isolate VR2332) and 35.5% with that of 
      European PRRSV (isolate LV), indicating that the PRRSV isolate was related to the 
      North American PRRSV genotype. The bacterially expressed N protein was used as a 
      target in a biopanning process using a phage display random peptide library. 
      Seven phages expressing different peptides had a specific binding activity with 
      the N protein. The putative binding motifs were identified by DNA sequencing. 
      More importantly, the selected phages harboring specific peptides that recognize 
      the N protein of PRRSV were able to efficiently distinguish PRRSV from other 
      viruses in enzyme-linked immunosorbent assays.
FAU - Ren, Xiaofeng
AU  - Ren X
AD  - College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai St., 
      Xiangfang District, 150030 Harbin, China.
FAU - Wang, Mingcui
AU  - Wang M
FAU - Yin, Jiechao
AU  - Yin J
FAU - Li, Guangxing
AU  - Li G
LA  - eng
SI  - GENBANK/GQ184823
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100317
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
RN  - 0 (Peptide Library)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Bacteriophages/*genetics
MH  - DNA, Viral/chemistry/genetics
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Molecular Sequence Data
MH  - *Peptide Library
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Protein Binding
MH  - Sequence Analysis, DNA
MH  - Viral Proteins/*metabolism
MH  - Virology/*methods
PMC - PMC2863871
EDAT- 2010/03/20 06:00
MHDA- 2010/08/07 06:00
PMCR- 2010/11/01
CRDT- 2010/03/19 06:00
PHST- 2010/03/19 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/08/07 06:00 [medline]
PHST- 2010/11/01 00:00 [pmc-release]
AID - JCM.01707-09 [pii]
AID - 1707-09 [pii]
AID - 10.1128/JCM.01707-09 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2010 May;48(5):1875-81. doi: 10.1128/JCM.01707-09. Epub 2010 
      Mar 17.