PMID- 20238274
OWN - NLM
STAT- MEDLINE
DCOM- 20100617
LR  - 20100318
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 638
DP  - 2010
TI  - Fluorescence in situ hybridization for molecular cytogenetic analysis in 
      filamentous fungi.
PG  - 235-57
LID - 10.1007/978-1-60761-611-5_18 [doi]
AB  - Fluorescence in situ hybridization (FISH) is a powerful technology for studying 
      eukaryotic chromosomes and genomes from the combined view of cytogenetics and 
      molecular biology, but its use in filamentous fungi has been limited. In this 
      chapter, we describe protocols to perform three basic FISH techniques in 
      filamentous fungi: (a) FISH mapping of unique sequences on the somatic 
      chromosomes and interphase nuclei, (b) chromosome painting to detect a specific 
      chromosome in the genome by fluorescent painting of the whole chromosome, and (c) 
      fiber FISH on the stretched DNA fibers for physical mapping. The ways of 
      preparing target specimens unique to filamentous fungi are included in the 
      protocols.
FAU - Tsuchiya, Dai
AU  - Tsuchiya D
AD  - Department of Biology, Indiana University, Bloomington, IN, USA.
FAU - Taga, Masatoki
AU  - Taga M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Fungal)
SB  - IM
MH  - *Chromosome Painting
MH  - Cytogenetic Analysis/*methods
MH  - DNA, Fungal/genetics
MH  - Fungi/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Hybridization/*methods
EDAT- 2010/03/20 06:00
MHDA- 2010/06/18 06:00
CRDT- 2010/03/19 06:00
PHST- 2010/03/19 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/06/18 06:00 [medline]
AID - 10.1007/978-1-60761-611-5_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;638:235-57. doi: 10.1007/978-1-60761-611-5_18.