PMID- 2024458
OWN - NLM
STAT- MEDLINE
DCOM- 19910604
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 182
IP  - 1
DP  - 1991 May
TI  - Properties of the human herpesvirus 6 strain Z29 genome: G + C content, length, 
      and presence of variable-length directly repeated terminal sequence elements.
PG  - 102-10
AB  - We have studied the structure of the human herpesvirus 6 (HHV-6) genome. The 
      density of genomic DNA is approximately 1.702 g/cm3 as determined by isopycnic 
      density gradient centrifugation, from which a mean G + C content of 43% was 
      calculated. The genomic termini were examined by exonuclease digestion and 
      DNA/DNA hybridization; relative molarities of restriction fragments were 
      determined by quantitative densitometry. The results indicate that the HHV-6(Z29) 
      genome has two unique termini and consists of a long unique segment bounded by a 
      directly repeated sequence element found in one copy at each end of the genome. 
      We estimated the length of the genome by pulsed-field gel electrophoresis and by 
      summation of restriction endonuclease fragment lengths. We observed two forms of 
      HHV-6(Z29) DNA of approximately 162 and 168 kb in length. The length 
      heterogeneity was localized within the terminal repeat element, each copy of 
      which is approximately 10.1 kb in length in the shorter form of the genome and 
      13.2 kb in length in the longer form of the genome.
FAU - Lindquester, G J
AU  - Lindquester GJ
AD  - Department of Biology, Rhodes College, Memphis, Tennessee 38112.
FAU - Pellett, P E
AU  - Pellett PE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - Base Composition
MH  - Blotting, Southern
MH  - Capsid/chemistry
MH  - DNA, Viral/*chemistry/genetics
MH  - Electrophoresis, Agar Gel
MH  - Herpesviridae/analysis/*genetics
MH  - Repetitive Sequences, Nucleic Acid
MH  - Restriction Mapping
MH  - Viral Core Proteins/chemistry
EDAT- 1991/05/01 00:00
MHDA- 1991/05/01 00:01
CRDT- 1991/05/01 00:00
PHST- 1991/05/01 00:00 [pubmed]
PHST- 1991/05/01 00:01 [medline]
PHST- 1991/05/01 00:00 [entrez]
AID - 0042-6822(91)90653-S [pii]
AID - 10.1016/0042-6822(91)90653-s [doi]
PST - ppublish
SO  - Virology. 1991 May;182(1):102-10. doi: 10.1016/0042-6822(91)90653-s.