PMID- 2026877
OWN - NLM
STAT- MEDLINE
DCOM- 19910611
LR  - 20210721
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 146
IP  - 10
DP  - 1991 May 15
TI  - Neutrophil attractant/activation protein-1 and monocyte chemoattractant protein-1 
      in rabbit. cDNA cloning and their expression in spleen cells.
PG  - 3483-8
AB  - Rabbit neutrophil attractant/activation protein-1 (NAP-1) and monocyte 
      chemoattractant protein-1 (MCP-1) were investigated. Rabbit spleen cells 
      stimulated with 5 micrograms/ml of Con A produced both neutrophil and monocyte 
      chemotactic activity. Physicochemical characteristics of those activities 
      obtained by HPLC gel filtration and HPLC chromatofocusing were very similar to 
      those of human NAP-1 and MCP-1, suggesting that rabbit spleen cells produce NAP-1 
      and MCP-1 after Con A stimulation. A cDNA library was constructed from mRNA 
      purified from Con A-stimulated rabbit spleen cells and screened with 
      oligonucleotide probes. By two rounds of screening, NAP-1 and MCP-1 cDNA were 
      cloned. NAP-1 cDNA comprises 1500 bp with an open reading frame that encodes for 
      a 101-amino acid protein highly similar to human NAP-1. MCP-1 cDNA comprises 607 
      bp with an open reading frame that encodes for a 124-amino acid protein highly 
      similar to human MCP-1. Expression of NAP-1 and MCP-1 mRNA by rabbit spleen cells 
      was studied. Both Con A- and LPS-stimulated spleen cells expressed NAP-1 and 
      MCP-1 mRNA, but the kinetics of expression were different. Con A rapidly induced 
      high NAP-1 and MCP-1 mRNA expression. LPS also rapidly induced NAP-1 mRNA 
      expression, but high MCP-1 mRNA expression was not observed until 15 h after 
      stimulation. Immunoprecipitation of metabolically labeled NAP-1 and MCP-1 with 
      anti-human NAP-1 or MCP-1 polyclonal antibodies was attempted. Immunoprecipitated 
      rabbit NAP-1 with a molecular mass of about 7 kDa was detected by SDS-PAGE and 
      radioautography, but MCP-1 was not. Cloned rabbit NAP-1 and MCP-1 will give us 
      opportunities to study the role of NAP-1 and MCP-1 in vivo.
FAU - Yoshimura, T
AU  - Yoshimura T
AD  - Immunopathology Section, National Cancer Institute-Frederick Cancer Research and 
      Development Center, Frederick, MD 21702.
FAU - Yuhki, N
AU  - Yuhki N
LA  - eng
SI  - GENBANK/M57439
SI  - GENBANK/M57440
SI  - GENBANK/M65297
SI  - GENBANK/M65298
SI  - GENBANK/M65299
SI  - GENBANK/M65300
SI  - GENBANK/M65301
SI  - GENBANK/M65302
SI  - GENBANK/M65303
SI  - GENBANK/M65304
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 11028-71-0 (Concanavalin A)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chemokine CCL2
MH  - Chemotactic Factors/analysis/*genetics/immunology
MH  - *Cloning, Molecular
MH  - Concanavalin A/pharmacology
MH  - Interleukin-8/analysis/*genetics/immunology
MH  - Molecular Sequence Data
MH  - Precipitin Tests
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Spleen/*chemistry
EDAT- 1991/05/15 00:00
MHDA- 1991/05/15 00:01
CRDT- 1991/05/15 00:00
PHST- 1991/05/15 00:00 [pubmed]
PHST- 1991/05/15 00:01 [medline]
PHST- 1991/05/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1991 May 15;146(10):3483-8.