PMID- 2027056
OWN - NLM
STAT- MEDLINE
DCOM- 19910607
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 11
IP  - 5
DP  - 1991 May
TI  - A monoclonal antibody that distinguishes between temporal and nasal retinal 
      axons.
PG  - 1470-7
AB  - A monoclonal antibody was developed that recognizes an antigen with an asymmetric 
      distribution in the chick retina. Immunohistochemistry showed that this antigen, 
      temporal retinal axon protein (TRAP), was present on most if not all axons that 
      arose from the temporal side of the retina. Very few of the axons from the nasal 
      side of the retina were positive for TRAP. The nasal-temporal difference appeared 
      to be in the number of axons that stained with this antibody rather than in the 
      intensity of staining. The transition between nasal and temporal retina based on 
      TRAP distribution appeared to be a vertical line centered on the optic fissure. A 
      competition-based ELISA was developed to quantify the average amount of TRAP on 
      axons in different regions of the retina. This assay also suggested that the 
      pattern of TRAP distribution across the retina was a step function, though the 
      results did not completely rule out the possibility of a continuous concentration 
      gradient oriented circumferentially around the retina. Explants of embryonic 
      nasal and temporal retina had a similar dichotomy in TRAP expression during the 
      first 1 or 2 d in culture. The antibody to TRAP bound to retinal neurites in 
      culture without the cell membrane being made permeable, which suggests that TRAP 
      is a cell-surface molecule. In culture, TRAP was also expressed on the growth 
      cones. Immunoblots showed that TRAP is trypsin sensitive and has an approximate 
      molecular mass of 135 kDa. This is the first molecule identified with an 
      asymmetric distribution in the nasal-temporal axis of the retina.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - McLoon, S C
AU  - McLoon SC
AD  - Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 
      55455.
LA  - eng
GR  - EYO5371/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Avian Proteins)
RN  - 0 (Eye Proteins)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - Antigens, Surface/analysis
MH  - *Avian Proteins
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eye Proteins/analysis
MH  - Immunoblotting
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Organ Culture Techniques
MH  - Retina/*cytology
MH  - Retinal Ganglion Cells/*cytology
PMC - PMC6575310
EDAT- 1991/05/01 00:00
MHDA- 1991/05/01 00:01
PMCR- 1991/11/01
CRDT- 1991/05/01 00:00
PHST- 1991/05/01 00:00 [pubmed]
PHST- 1991/05/01 00:01 [medline]
PHST- 1991/05/01 00:00 [entrez]
PHST- 1991/11/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.11-05-01470.1991 [doi]
PST - ppublish
SO  - J Neurosci. 1991 May;11(5):1470-7. doi: 10.1523/JNEUROSCI.11-05-01470.1991.