PMID- 20298763
OWN - NLM
STAT- MEDLINE
DCOM- 20100820
LR  - 20131121
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 168
IP  - 1
DP  - 2010 Jun 16
TI  - MDMA causes a redistribution of serotonin transporter from the cell surface to 
      the intracellular compartment by a mechanism independent of phospho-p38-mitogen 
      activated protein kinase activation.
PG  - 82-95
LID - 10.1016/j.neuroscience.2010.03.018 [doi]
AB  - 3,4-methylenedioxymethamphetamine (MDMA) causes long-term serotonin depletion and 
      reduced serotonin transporter (SERT) function in humans and in animal models. 
      Using quantitative Western blotting and real-time PCR, we have shown that total 
      SERT protein in the striatum and nucleus accumbens and mRNA levels in the dorsal 
      raphe nucleus were not significantly changed following MDMA exposure in rats (4 x 
      2 h i.p. injections, 10 mg/kg each). In mouse neuroblastoma (N(2)A) cells 
      transiently expressing green fluorescent protein-tagged human SERT (GFP-hSERT), 
      we have shown redistribution of SERT from the cell surface to intracellular 
      vesicles on exposure to MDMA using cell surface biotinylation, total internal 
      reflection fluorescence microscopy (TIRFM) and live-cell confocal microscopy. To 
      investigate the mechanism responsible for SERT redistribution, we used specific 
      antibodies to phospho-p38-mitogen activated protein kinase (p38 MAPK), a known 
      signalling pathway involved in SERT membrane expression. We found that p38 MAPK 
      activation was not involved in the MDMA-induced redistribution of SERT from the 
      cell-surface to the cell interior. A loss of SERT from the cell surface on acute 
      exposure to MDMA may contribute to the decreased SERT function seen in rats 
      exposed to MDMA.
CI  - 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Kivell, B
AU  - Kivell B
AD  - School of Biological Sciences, Victoria University of Wellington, Kelburn Parade, 
      Wellington, New Zealand. bronwyn.kivell@vuw.ac.nz
FAU - Day, D
AU  - Day D
FAU - Bosch, P
AU  - Bosch P
FAU - Schenk, S
AU  - Schenk S
FAU - Miller, J
AU  - Miller J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100315
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain/drug effects/metabolism
MH  - Cell Line, Tumor
MH  - Cell Membrane/metabolism
MH  - Enzyme Activation
MH  - Male
MH  - Mice
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*toxicity
MH  - Phosphorylation
MH  - Protein Transport
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serotonin Plasma Membrane Transport Proteins/*metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2010/03/20 06:00
MHDA- 2010/08/21 06:00
CRDT- 2010/03/20 06:00
PHST- 2009/11/11 00:00 [received]
PHST- 2010/03/08 00:00 [revised]
PHST- 2010/03/09 00:00 [accepted]
PHST- 2010/03/20 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/08/21 06:00 [medline]
AID - S0306-4522(10)00369-6 [pii]
AID - 10.1016/j.neuroscience.2010.03.018 [doi]
PST - ppublish
SO  - Neuroscience. 2010 Jun 16;168(1):82-95. doi: 10.1016/j.neuroscience.2010.03.018. 
      Epub 2010 Mar 15.