PMID- 20298805
OWN - NLM
STAT- MEDLINE
DCOM- 20101008
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 701
IP  - 1
DP  - 2010 Aug 14
TI  - Relationship between chromatin structure, DNA damage and repair following 
      X-irradiation of human lymphocytes.
PG  - 86-91
LID - 10.1016/j.mrgentox.2010.03.005 [doi]
AB  - Earlier studies using the technique of premature chromosome condensation (PCC) 
      have shown that in human lymphocytes, exchange type of aberrations are formed 
      immediately following low doses (<2 Gy) of X-rays, whereas at higher doses these 
      aberrations increase with the duration of recovery. This reflects the relative 
      roles of slow and fast repair in the formation of exchange aberrations. The 
      underlying basis for slow and fast repairing components of the DNA repair may be 
      related to differential localization of the initial damage in the genome, i.e., 
      between relaxed and condensed chromatin. We have tried to gain some insight into 
      this problem by (a) X-irradiating lymphocytes in the presence of dimethyl 
      sulfoxide (DMSO) a potent scavenger of radiation-induced .OH radicals followed by 
      PCC and (b) probing the damage and repair in two specific chromosomes, 18 and 19, 
      which are relatively poor and rich in transcribing genes by COMET-FISH, a 
      combination of Comet assay and fluorescence in situ hybridization (FISH) 
      techniques. Results obtained show (a) that both fast appearing and slowly formed 
      exchange aberrations seem to take place in relaxed chromatin, since they are 
      affected to a similar extent by DMSO, (b) significant differential DNA breakage 
      of chromosome 18 compared to chromosome 19 in both G0 and G1 phases of the cell 
      cycle as detected by Comet assay, indicating that relaxed chromatin containing 
      high densities of transcriptionally active genes shows less fragmentation due to 
      fast repair (chromosome 19) compared to chromosome 18, and (c) that relaxed 
      chromatin is repaired or mis-repaired faster than more compact chromatin.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Mosesso, Pasquale
AU  - Mosesso P
AD  - Dipartimento di Agrobiologia e Agrochimica, Universita degli Studi della Tuscia, 
      Via San Camillo de Lellis s.n.c., 01100 Viterbo, Italy. mosesso@unitus.it
FAU - Palitti, Fabrizio
AU  - Palitti F
FAU - Pepe, Gaetano
AU  - Pepe G
FAU - Pinero, Joaquin
AU  - Pinero J
FAU - Bellacima, Raffaella
AU  - Bellacima R
FAU - Ahnstrom, Gunnar
AU  - Ahnstrom G
FAU - Natarajan, Adayapalam T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
DEP - 20100316
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Chromatin)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Chromatin/*chemistry
MH  - *DNA Damage
MH  - *DNA Repair
MH  - Dimethyl Sulfoxide/pharmacology
MH  - Humans
MH  - Lymphocytes/radiation effects
MH  - Male
MH  - X-Rays/*adverse effects
EDAT- 2010/03/20 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/03/20 06:00
PHST- 2010/03/05 00:00 [received]
PHST- 2010/03/09 00:00 [accepted]
PHST- 2010/03/20 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
AID - S1383-5718(10)00109-9 [pii]
AID - 10.1016/j.mrgentox.2010.03.005 [doi]
PST - ppublish
SO  - Mutat Res. 2010 Aug 14;701(1):86-91. doi: 10.1016/j.mrgentox.2010.03.005. Epub 
      2010 Mar 16.