PMID- 20298859
OWN - NLM
STAT- MEDLINE
DCOM- 20101026
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 81
IP  - 3
DP  - 2010 May 15
TI  - Pseudo-homogeneous immunoextraction of epitestosterone from human urine samples 
      based on gold-coated magnetic nanoparticles.
PG  - 819-23
LID - 10.1016/j.talanta.2010.01.021 [doi]
AB  - A pseudo-homogeneous immunoextraction method based on gold-coated magnetic 
      nanoparticles (MNPs) for the specific extraction and quantitative analysis of 
      epitestosterone (17alpha-hydroxy-4-androsten-3-one, abbreviated as "ET") from 
      human urine samples by high-performance liquid chromatography (HPLC) has been 
      developed. Half-IgG of anti-ET monoclonal antibodies were covalently immobilized 
      onto (Fe(3)O(4))(core)-Au(shell) (Fe(3)O(4)@Au) MNPs. An external magnetic field 
      was applied to collect the MNPs which were then rinsed with distilled water 
      followed by elution with absolute methanol to obtain ET as the analyte. The 
      obtained extraction solution was analyzed by HPLC with UV detection (244nm) 
      within 12min. The standard calibration curve for ET showed good linearity in the 
      range of 20-200ngmL(-1) in phosphate-buffered saline (PBS) solutions with 
      acceptable accuracy and precision. Limit of detection for ET was 0.06ngmL(-1) due 
      to an enrichment factor of 100-fold was achieved. The results obtained by the 
      present method for spiked human urine samples were in agreement with those from 
      indirect competitive enzyme-linked immunoadsorbent assays (ELISAs). The 
      antibody-conjugated Fe(3)O(4)@Au MNPs are novel materials for immunoaffinity 
      extraction. Compared with the conventional technique using immunoaffinity column, 
      the method described here for sample pretreatment was fast, highly specific, and 
      easy to operate.
FAU - Qiu, Shuang
AU  - Qiu S
AD  - Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of 
      Biochemistry and Molecular Engineering of Ministry of Education, Institute of 
      Analytical Chemistry, College of Chemistry, Peking University, Beijing, China.
FAU - Xu, Li
AU  - Xu L
FAU - Cui, Yi-Ran
AU  - Cui YR
FAU - Deng, Qin-Pei
AU  - Deng QP
FAU - Wang, Wei
AU  - Wang W
FAU - Chen, Hong-Xu
AU  - Chen HX
FAU - Zhang, Xin-Xiang
AU  - Zhang XX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100121
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Immunoglobulin G)
RN  - 481-30-1 (Epitestosterone)
RN  - 7440-57-5 (Gold)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/methods
MH  - *Enzyme Assays
MH  - Epitestosterone/chemistry/*urine
MH  - Gold/*chemistry
MH  - Humans
MH  - Immunoglobulin G/chemistry
MH  - Magnetics
MH  - Metal Nanoparticles/*chemistry
MH  - Methanol/chemistry
MH  - Nanotechnology/*methods
MH  - Reproducibility of Results
MH  - Ultraviolet Rays
MH  - Urinalysis/*methods
EDAT- 2010/03/20 06:00
MHDA- 2010/10/27 06:00
CRDT- 2010/03/20 06:00
PHST- 2009/11/24 00:00 [received]
PHST- 2010/01/12 00:00 [revised]
PHST- 2010/01/13 00:00 [accepted]
PHST- 2010/03/20 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/10/27 06:00 [medline]
AID - S0039-9140(10)00035-4 [pii]
AID - 10.1016/j.talanta.2010.01.021 [doi]
PST - ppublish
SO  - Talanta. 2010 May 15;81(3):819-23. doi: 10.1016/j.talanta.2010.01.021. Epub 2010 
      Jan 21.