PMID- 20299167
OWN - NLM
STAT- MEDLINE
DCOM- 20100818
LR  - 20100426
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 198
IP  - 1-3
DP  - 2010 May 20
TI  - Determination of a new designer drug, N-hydroxy-3,4-methylenedioxymethamphetamine 
      and its metabolites in rats using ultra-performance liquid chromatography-tandem 
      mass spectrometry.
PG  - 62-9
LID - 10.1016/j.forsciint.2010.02.013 [doi]
AB  - An N-hydroxy analogue of 3,4-methylendioxymethamphetamine (MDMA), N-hydroxy MDMA 
      (N-OH MDMA), has recently been distributed as a new designer drug in some drug 
      markets. Very little data is available to the metabolic and pharmacological 
      properties of N-OH MDMA, although it has been reported that the N-demethyl 
      analogue, N-hydroxy-3,4-methylenedioxyamphetamine (N-OH MDA), is mainly 
      metabolized to MDA in rats. In this study, an analytical method for the 
      determination of N-OH MDMA and its metabolites in biological samples was 
      developed, and the metabolic properties of N-OH MDMA in rats were investigated. 
      After the i.p. administration of N-OH MDMA to pigmented hairy rats (5mg/kg/day, 
      10 days), N-OH MDMA and its N-dehydroxy and N-demethyl metabolites (MDMA, N-OH 
      MDA and MDA) in rat plasma, urine and hair samples were determined by 
      ultra-performance LC (UPLC)-MS/MS. The hair sample was extracted by 1-h 
      sonication and overnight soaking in 5M hydrochloric acid-methanol (1:20). The 
      plasma, urine, and hair extract samples were purified using a solid-phase 
      extraction procedure. N-OH MDMA in the samples could be precisely analyzed by 
      avoiding an alkaline environment. The parent compound very rapidly disappeared 
      from the rat plasma (<15min) and urine (<10h), and most of the N-OH MDMA was 
      excreted in the rat urine as MDMA and MDA in 72h. In the rat hair samples 
      collected 4 weeks after the first administration, N-OH MDMA (0.03ng/mg) and N-OH 
      MDA (0.13ng/mg) were clearly detected as well as MDMA (149ng/mg) and MDA 
      (52ng/mg). This analytical method will be useful for the analysis of N-OH MDMA 
      and its metabolites in biological samples.
FAU - Kikura-Hanajiri, Ruri
AU  - Kikura-Hanajiri R
AD  - National Institute of Health Sciences, Setagaya, Tokyo, Japan. kikura@nihs.go.jp
FAU - Kawamura, Maiko
AU  - Kawamura M
FAU - Miyajima, Atsuko
AU  - Miyajima A
FAU - Sunouchi, Momoko
AU  - Sunouchi M
FAU - Goda, Yukihiro
AU  - Goda Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100317
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Designer Drugs)
RN  - 0 (Hallucinogens)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - 74698-47-8 (N-hydroxy-1-(3,4-methylenedioxyphenyl)-2-aminopropane)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*analogs & derivatives/analysis
MH  - Animals
MH  - Chromatography, Liquid
MH  - Designer Drugs/*analysis
MH  - Forensic Toxicology
MH  - Hair/chemistry
MH  - Hallucinogens/*analysis
MH  - Male
MH  - Rats
MH  - Tandem Mass Spectrometry
EDAT- 2010/03/20 06:00
MHDA- 2010/08/19 06:00
CRDT- 2010/03/20 06:00
PHST- 2009/08/21 00:00 [received]
PHST- 2010/01/29 00:00 [revised]
PHST- 2010/02/09 00:00 [accepted]
PHST- 2010/03/20 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/08/19 06:00 [medline]
AID - S0379-0738(10)00065-4 [pii]
AID - 10.1016/j.forsciint.2010.02.013 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2010 May 20;198(1-3):62-9. doi: 
      10.1016/j.forsciint.2010.02.013. Epub 2010 Mar 17.