PMID- 20300744
OWN - NLM
STAT- MEDLINE
DCOM- 20100618
LR  - 20211020
IS  - 1432-1424 (Electronic)
IS  - 0022-2631 (Linking)
VI  - 234
IP  - 2
DP  - 2010 Apr
TI  - Nonenzymatic augmentation of lactate transport via monocarboxylate transporter 
      isoform 4 by carbonic anhydrase II.
PG  - 125-35
LID - 10.1007/s00232-010-9240-y [doi]
AB  - Monocarboxylate transporters (MCTs) are carriers of high-energy metabolites like 
      lactate and pyruvate, and different MCT isoforms are expressed in a wide range of 
      cells and tissues. Transport activity of MCT isoform 1 (MCT1), heterologously 
      expressed in Xenopus oocytes, has previously been shown to be supported by 
      carbonic anhydrase II (CAII) in a noncatalytic manner. In the present study, we 
      investigated possible interactions of CAII with MCT4, expressed in Xenopus 
      oocytes. MCT4 transport activity is enhanced both by injected and by coexpressed 
      CAII, similar to MCT1, with the highest augmentation at low extracellular pH and 
      low lactate concentrations. CAII-induced augmentation in MCT4 transport activity 
      is independent from the enzyme's catalytic function, as shown by application of 
      the CA inhibitor ethoxyzolamide and by coexpression of MCT4 with the 
      catalytically inactive mutant CAII-V143Y.
FAU - Becker, Holger M
AU  - Becker HM
AD  - Arbeitsgruppe Zoologie/Membrantransport, FB Biologie, TU Kaiserslautern, P.O. Box 
      3049, 67653, Kaiserslautern, Germany. h.becker@biologie.uni-kl.de
FAU - Klier, Michael
AU  - Klier M
FAU - Deitmer, Joachim W
AU  - Deitmer JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100319
PL  - United States
TA  - J Membr Biol
JT  - The Journal of membrane biology
JID - 0211301
RN  - 0 (Carbonic Anhydrase Inhibitors)
RN  - 0 (Monocarboxylic Acid Transporters)
RN  - 0 (Muscle Proteins)
RN  - 0 (Slc16a3 protein, rat)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
RN  - Z52H4811WX (Ethoxzolamide)
SB  - IM
MH  - Animals
MH  - Carbonic Anhydrase II/*metabolism
MH  - Carbonic Anhydrase Inhibitors/pharmacology
MH  - Catalysis
MH  - Ethoxzolamide/pharmacology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Lactic Acid/*metabolism
MH  - Monocarboxylic Acid Transporters/*metabolism
MH  - Muscle Proteins/*metabolism
MH  - Oocytes/metabolism
MH  - Rats
MH  - Xenopus laevis
EDAT- 2010/03/20 06:00
MHDA- 2010/06/19 06:00
CRDT- 2010/03/20 06:00
PHST- 2009/09/04 00:00 [received]
PHST- 2010/02/26 00:00 [accepted]
PHST- 2010/03/20 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/06/19 06:00 [medline]
AID - 10.1007/s00232-010-9240-y [doi]
PST - ppublish
SO  - J Membr Biol. 2010 Apr;234(2):125-35. doi: 10.1007/s00232-010-9240-y. Epub 2010 
      Mar 19.