PMID- 20301101
OWN - NLM
STAT- MEDLINE
DCOM- 20100622
LR  - 20151119
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 24
IP  - 8
DP  - 2010 Apr 30
TI  - Direct quantification of 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid 
      in urine by liquid chromatography/tandem mass spectrometry in relation to doping 
      control analysis.
PG  - 1133-41
LID - 10.1002/rcm.4499 [doi]
AB  - An accurate and precise method for the quantification of 
      11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid (THCA) in urine by liquid 
      chromatography/tandem mass spectrometry (LC/MS/MS) for doping analysis purposes 
      has been developed. The method involves the use of only 200 microL of urine and 
      the use of D(9)-THCA as internal standard. No extraction procedure is used. The 
      urine samples are hydrolysed using sodium hydroxide and diluted with a mixture of 
      methanol/glacial acetic acid (1:1). Chromatographic separation is achieved using 
      a C8 column with gradient elution. All MS and MS/MS parameters were optimised in 
      both positive and negative electrospray ionisation modes. For the identification 
      and the quantification of THCA three product ions are monitored in both 
      ionisation modes. The method is linear over the studied range (5-40 ng/mL), with 
      satisfactory intra-and inter-assay precision, and the relative standard 
      deviations (RSDs) are lower than 15%. Good accuracy is achieved with bias less 
      than 10% at all levels tested. No significant matrix effects are observed. The 
      selectivity and specificity are satisfactory, and no interferences are detected. 
      The LC/MS/MS method was applied for the analysis of 48 real urine samples 
      previously analysed with a routine gas chromatography/mass spectrometry (GC/MS) 
      method. A good correlation between the two methods was obtained (r(2) > 0.98) 
      with a slope close to 1.
CI  - Copyright (c) 2010 John Wiley & Sons, Ltd.
FAU - Chebbah, C
AU  - Chebbah C
AD  - Doping Control Laboratory, Department of Clinical Biology, Microbiology and 
      Immunology, Ghent University - UGent, Technologiepark 30, B-9052 Zwijnaarde, 
      Belgium. charaf.chebbah@UGent.be
FAU - Pozo, O J
AU  - Pozo OJ
FAU - Deventer, K
AU  - Deventer K
FAU - Van Eenoo, P
AU  - Van Eenoo P
FAU - Delbeke, F T
AU  - Delbeke FT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 4TPC9E4A32 (11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid)
RN  - 7J8897W37S (Dronabinol)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - *Doping in Sports
MH  - Dronabinol/*analogs & derivatives/chemistry/urine
MH  - Humans
MH  - Linear Models
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2010/03/20 06:00
MHDA- 2010/06/23 06:00
CRDT- 2010/03/20 06:00
PHST- 2010/03/20 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/06/23 06:00 [medline]
AID - 10.1002/rcm.4499 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2010 Apr 30;24(8):1133-41. doi: 10.1002/rcm.4499.