PMID- 20301200
OWN - NLM
STAT- MEDLINE
DCOM- 20100517
LR  - 20100427
IS  - 1531-4995 (Electronic)
IS  - 0023-852X (Linking)
VI  - 120
IP  - 5
DP  - 2010 May
TI  - PHR1 is a vesicle-bound protein abundantly expressed in mature olfactory neurons.
PG  - 1002-10
LID - 10.1002/lary.20779 [doi]
AB  - OBJECTIVES/HYPOTHESIS: To characterize the role of Phr1, a gene highly expressed 
      in primary sensory neurons where it encodes an integral membrane protein with an 
      N-terminal pleckstrin homology domain and a C-terminal transmembrane domain, in 
      the olfactory system. METHODS: We studied the immunelocalization of the PHR1 
      protein in mouse olfactory epithelium both at steady state and during 
      regeneration following methyl bromide (MeBr) exposure using scanning confocal 
      microscopy. Additionally, we examined the electrophysiologic role of Phr1 in 
      olfaction and short-term olfactory adaptation. RESULTS: We found that PHR1 is 
      abundantly and specifically expressed in olfactory neurons. It is widely 
      distributed in punctate, vesiculated organelles throughout the cell bodies, 
      axons, and glomeruli of primary olfactory neurons but is specifically excluded 
      from the olfactory cilia. In the regenerating olfactory epithelium, PHR1 
      expression appears at 14 days following MeBr ablation coinciding with the onset 
      of olfactory neuron maturity. Despite the abundant and specific expression 
      throughout the olfactory neurons, mice lacking Phr1 did not exhibit differences 
      in the distribution of the components of olfactory signal transduction system, 
      the rate of olfactory regeneration following MeBr exposure, olfactory function, 
      or short-term adaptation to odors. CONCLUSIONS: Phr1 is widely and abundantly 
      expressed throughout mature olfactory neurons and other primary sensory neurons, 
      but its absence does not appear to affect olfactory morphology, regeneration, 
      sensory function, or adaptation. The exact function of Phr1 remains to be 
      discovered.
FAU - Tan, Bruce
AU  - Tan B
AD  - Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University 
      School of Medicine, Baltimore, MD, USA.
FAU - Brown, David
AU  - Brown D
FAU - Xu, Shunbin
AU  - Xu S
FAU - Valle, David
AU  - Valle D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (PRH1 protein, human)
RN  - 0 (Salivary Proline-Rich Proteins)
SB  - IM
MH  - Animals
MH  - Gene Expression/genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Microscopy, Confocal
MH  - Microscopy, Electron
MH  - Microscopy, Fluorescence
MH  - Nerve Regeneration/genetics
MH  - Olfactory Mucosa/*metabolism
MH  - Olfactory Nerve/anatomy & histology/*metabolism
MH  - Olfactory Receptor Neurons/*metabolism/ultrastructure
MH  - Salivary Proline-Rich Proteins/*genetics
MH  - Signal Transduction/genetics
EDAT- 2010/03/20 06:00
MHDA- 2010/05/18 06:00
CRDT- 2010/03/20 06:00
PHST- 2010/03/20 06:00 [entrez]
PHST- 2010/03/20 06:00 [pubmed]
PHST- 2010/05/18 06:00 [medline]
AID - 10.1002/lary.20779 [doi]
PST - ppublish
SO  - Laryngoscope. 2010 May;120(5):1002-10. doi: 10.1002/lary.20779.