PMID- 20302682
OWN - NLM
STAT- MEDLINE
DCOM- 20100809
LR  - 20131121
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 104
IP  - 2
DP  - 2010 Jul
TI  - Quercetin, the active phenolic component in kiwifruit, prevents hydrogen 
      peroxide-induced inhibition of gap-junction intercellular communication.
PG  - 164-70
LID - 10.1017/S0007114510000346 [doi]
AB  - We evaluated the effects of the two main kiwifruit cultivars (gold kiwifruit 
      (GOK) and green kiwifruit (GRK)) and their active phenolic compound, quercetin, 
      on H2O2-induced inhibition of gap-junction intercellular communication (GJIC) in 
      WB-F344 rat liver epithelial cells. We found that both GOK and GRK protect 
      WB-F344 cells from H2O2-induced inhibition of GJIC. The extracellular 
      signal-regulated protein kinase 1/2 (ERK1/2)-connexin 43 (Cx43) signalling 
      pathway is crucial for the regulation of GJIC, and both GOK and GRK blocked the 
      H2O2-induced phosphorylation of Cx43 and ERK1/2 in WB-F344 cells. Quercetin alone 
      attenuated the H2O2-mediated ERK1/2-Cx43 signalling pathway and consequently 
      reversed H2O2-mediated inhibition of GJIC in WB-F344 cells. A free 
      radical-scavenging assay using 1,1-diphenyl-2-picrylhydrazyl showed that the 
      scavenging activity of quercetin was higher than that of a synthetic antioxidant, 
      butylated hydroxytoluene, per mol, suggesting that the chemopreventive effect of 
      quercetin on H2O2-mediated inhibition of ERK1/2-Cx43 signalling and GJIC may be 
      mediated through its free radical-scavenging activity. Since the carcinogenicity 
      of reactive oxygen species such as H2O2 is attributable to the inhibition of 
      GJIC, GOK, GRK and quercetin may have chemopreventive potential by preventing the 
      inhibition of GJIC.
FAU - Lee, Dong Eun
AU  - Lee DE
AD  - Major in Biomodulation, WCU, Department of Agricultural Biotechnology, Research 
      Institute for Agriculture and Life Sciences, Seoul National University, Seoul 
      151-921, Republic of Korea.
FAU - Shin, Bong Jik
AU  - Shin BJ
FAU - Hur, Haeng Jeon
AU  - Hur HJ
FAU - Kim, Jong Hun
AU  - Kim JH
FAU - Kim, Jiyoung
AU  - Kim J
FAU - Kang, Nam Joo
AU  - Kang NJ
FAU - Kim, Dae Ok
AU  - Kim DO
FAU - Lee, Chang Yong
AU  - Lee CY
FAU - Lee, Ki Won
AU  - Lee KW
FAU - Lee, Hyong Joo
AU  - Lee HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100322
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (Connexin 43)
RN  - 0 (Plant Extracts)
RN  - 9IKM0I5T1E (Quercetin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Actinidia/*chemistry/classification
MH  - Animals
MH  - Cell Communication/*drug effects
MH  - Cell Line
MH  - Connexin 43/metabolism
MH  - Epithelial Cells/drug effects/physiology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Fruit/*chemistry
MH  - Gap Junctions/*drug effects
MH  - Hydrogen Peroxide/*pharmacology
MH  - Liver/cytology
MH  - Phosphorylation
MH  - Plant Extracts/chemistry/pharmacology
MH  - Quercetin/chemistry/*pharmacology
MH  - Rats
EDAT- 2010/03/23 06:00
MHDA- 2010/08/10 06:00
CRDT- 2010/03/23 06:00
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/08/10 06:00 [medline]
AID - S0007114510000346 [pii]
AID - 10.1017/S0007114510000346 [doi]
PST - ppublish
SO  - Br J Nutr. 2010 Jul;104(2):164-70. doi: 10.1017/S0007114510000346. Epub 2010 Mar 
      22.