PMID- 20302795
OWN - NLM
STAT- MEDLINE
DCOM- 20160423
LR  - 20181201
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 31
IP  - 2
DP  - 2010 Feb
TI  - [A novel single nucleotide polymorphism-based method for quantitative assessment 
      of chimerism after allogeneic stem cell transplantation.].
PG  - 92-6
AB  - OBJECTIVE: To develop a novel single nucleotide polymorphism (SNP)-PCR based 
      method for quantitative detection of chimerism after allogeneic haemopoietic stem 
      cell transplantation (allo-HSCT), and to explore its feasibility, accuracy and 
      superiority. METHODS: 18 SNP loci were sereened to identify informative markers 
      for detecting chimerism in each donor/recipient pair before transplantation. Then 
      the chimerism rate of each informative marker was analyzed by real-time 
      quantitative PCR (RQ-PCR). The accuracy and sensitivity were verified by multiple 
      proportion dilution and analogy chimerism compared with quantitative detection of 
      short tandem repeat (STR)-PCR, fluorescence in situ hybridization (FISH) and 
      fusion gene. RESULTS: (1) The average slope of the 17 time amplications of the 
      internal control plasmid was -3.39, the average intercept was 39.97, correlation 
      coefficients were more than 0.995, which was close to the theoretical level. The 
      intra- and interassay variability was 0.50% and 1.1%, respectively, which were 
      both in the allowed ranges. A linear correlation with artificial mixed chimerism 
      is above 0.99 and a sensitivity of 0.01% proved reproducible. (2) At least one 
      informative marker could be found in over 95% of 40 donor/recipient pairs. The 
      results of the chimerisms derived from SNP-PCR were consistent with that from 
      STR-PCR (96.7%), FISH and fusion gene analasis (P > 0.05); the quantitative 
      results of special fusion gene transcripts were negtive in complete chimerism 
      samples, and positive in mixed chimerism samples. CONCLUSIONS: This new assay 
      which overcome the PCR competition and plateau biases of STR-PCR provides an 
      accurate, reliable and rapid quantitative assessment of mixed chimerism after 
      allo-transplantation. It is highly promising for of clinical application and may 
      take the place of STR-PCR in the conventional chimerisim assessment.
FAU - Shao, Yu
AU  - Shao Y
AD  - Department of Hematology, Chang Hai Hospital, The Second Military Medical 
      University, Shanghai 200433, China.
FAU - Wang, Jian-Min
AU  - Wang JM
FAU - Gong, Sheng-Lan
AU  - Gong SL
FAU - Cai, Zai-Long
AU  - Cai ZL
FAU - Zhang, Wei-Ping
AU  - Zhang WP
FAU - Song, Xian-Min
AU  - Song XM
FAU - Wang, Li-Ping
AU  - Wang LP
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - *Chimerism
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Polymorphism, Single Nucleotide
MH  - Stem Cell Transplantation
MH  - *Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 2010/03/23 06:00
MHDA- 2016/04/24 06:00
CRDT- 2010/03/23 06:00
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2016/04/24 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2010 Feb;31(2):92-6.