PMID- 20303011
OWN - NLM
STAT- MEDLINE
DCOM- 20100402
LR  - 20100322
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 198
IP  - 1
DP  - 2010 Apr 1
TI  - Genomic alterations of chromosome region 11p as predictive marker by array 
      comparative genomic hybridization in lung adenocarcinoma patients.
PG  - 27-34
LID - 10.1016/j.cancergencyto.2009.12.001 [doi]
AB  - Array comparative genomic hybridization (aCGH) provides a method to 
      quantitatively measure the changes of DNA copy number with an extremely high 
      resolution and to map them directly onto the complete linear genome sequences. In 
      this study, we used aCGH to compare genomic alterations in fresh-frozen lung 
      cancer tissues of 21 adenocarcinomas (AdCCs) (11 early relapse and 10 nonrelapse) 
      and identified genomic alterations that showed significant by different frequency 
      between early relapse and nonrelapse AdCCs. Twelve clones were identified by the 
      false discovery rate (FDR) test, and Kaplan-Meier analyses were selected as 
      predictive markers. The significant gain clones were found in 11p (11p15.4, 
      11p15.1, and 11p13). When the cutoff value was 2, study of the association 
      between candidate clones and relapse prediction revealed that early relapse and 
      nonrelapse groups were most effectively separated. To further validate the gain 
      of chromosome 11p region that was identified by array CGH, fluorescence in situ 
      hybridization (FISH) was performed. To further confirm the results of aCGH, copy 
      number changes of cancer-related candidate genes in AdCC patients were compared 
      by real-time quantitative polymerase chain reaction. Array CGH and real-time 
      quantitative polymerase chain reaction data were found to correspond to 
      delineated DNA copy number changes. Genomic alterations of chromosome 11p region 
      in AdCC patients were observed with aCGH, and a relapsable marker was identified 
      in the nonrelapse group. This marker could be useful in stratifying patient 
      groups according to likelihood of relapse for adjuvant treatment after surgical 
      resection.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Sung, Jae Sook
AU  - Sung JS
AD  - Genomic Research Center for Lung and Breast/Ovarian Cancers, Korea University 
      Anam Hospital, Seongbuk-gu, Seoul, Republic of Korea.
FAU - Park, Kyong Hwa
AU  - Park KH
FAU - Kim, Yeul Hong
AU  - Kim YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 11
MH  - Comparative Genomic Hybridization/*methods
MH  - *DNA Copy Number Variations
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - Recurrence
EDAT- 2010/03/23 06:00
MHDA- 2010/04/03 06:00
CRDT- 2010/03/23 06:00
PHST- 2009/07/28 00:00 [received]
PHST- 2009/11/10 00:00 [revised]
PHST- 2009/12/05 00:00 [accepted]
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/04/03 06:00 [medline]
AID - S0165-4608(09)00691-8 [pii]
AID - 10.1016/j.cancergencyto.2009.12.001 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2010 Apr 1;198(1):27-34. doi: 
      10.1016/j.cancergencyto.2009.12.001.