PMID- 20303688
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20131121
IS  - 1618-0984 (Electronic)
IS  - 0723-2020 (Linking)
VI  - 33
IP  - 3
DP  - 2010 Apr
TI  - Arsenic-resistant bacteria associated with roots of the wild Cirsium arvense (L.) 
      plant from an arsenic polluted soil, and screening of potential plant 
      growth-promoting characteristics.
PG  - 154-64
LID - 10.1016/j.syapm.2010.02.004 [doi]
AB  - A rhizobacterial community, associated with the roots of wild thistle Cirsium 
      arvense (L.) growing in an arsenic polluted soil, was studied by fluorescence in 
      situ hybridization (FISH) analysis in conjunction with cultivation-based methods. 
      In the bulk, rhizosphere, and rhizoplane fractions of the soil, the qualitative 
      picture obtained by FISH analysis of the main phylogenetic bacterial groups was 
      similar and was predominantly comprised of Alphaproteobacteria, 
      Betaproteobacteria, and Gammaproteobacteria. The arsenic-resistant isolates 
      belonged to 13 genera, the most abundant being those of Bacillus, Achromobacter, 
      Brevundimonas, Microbacterium, and Ochrobactrum. Most bacteria grew in the 
      presence of high arsenic concentrations (over 100mM arsenate and 10mM arsenite). 
      Most strains possessed the ArsC, ArsB and ACR3 genes homologous to arsenate 
      reductase and to the two classes of arsenite efflux pumps, respectively, peculiar 
      to the ars operon of the arsenic detoxification system. ArsB and ACR3 were 
      present simultaneously in highly resistant strains. An inconsistency between 16S 
      rRNA phylogenetic affiliations and the arsenate reductase sequences of the 
      strains was observed, indicating possible horizontal transfer of arsenic 
      resistance genes in the soil bacterial community. Several isolates were able to 
      reduce arsenate and to oxidise arsenite. In particular, Ancylobacter 
      dichloromethanicum strain As3-1b possessed both characteristics, and arsenite 
      oxidation occurred in the strain also under chemoautotrophic conditions. Some 
      rhizobacteria produced siderophores, indole acetic acid and 
      1-amino-cyclopropane-1-carboxylic acid deaminase, thus possessing potential plant 
      growth-promoting traits.
FAU - Cavalca, Lucia
AU  - Cavalca L
AD  - Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Universita 
      degli Studi di Milano, Via Celoria 2, Milano-20133, Italy.
FAU - Zanchi, Raffaella
AU  - Zanchi R
FAU - Corsini, Anna
AU  - Corsini A
FAU - Colombo, Milena
AU  - Colombo M
FAU - Romagnoli, Cristina
AU  - Romagnoli C
FAU - Canzi, Enrica
AU  - Canzi E
FAU - Andreoni, Vincenza
AU  - Andreoni V
LA  - eng
SI  - GENBANK/FN392622
SI  - GENBANK/FN392623
SI  - GENBANK/FN392624
SI  - GENBANK/FN392625
SI  - GENBANK/FN392626
SI  - GENBANK/FN392627
SI  - GENBANK/FN392628
SI  - GENBANK/FN392629
SI  - GENBANK/FN392630
SI  - GENBANK/FN392631
SI  - GENBANK/FN392632
SI  - GENBANK/FN392633
SI  - GENBANK/FN392634
SI  - GENBANK/FN392635
SI  - GENBANK/FN392636
SI  - GENBANK/FN392637
SI  - GENBANK/FN392638
SI  - GENBANK/FN392639
SI  - GENBANK/FN392640
SI  - GENBANK/FN392641
SI  - GENBANK/FN392642
SI  - GENBANK/FN392643
SI  - GENBANK/FN392644
SI  - GENBANK/FN392645
SI  - GENBANK/FN392646
SI  - GENBANK/FN392647
SI  - GENBANK/FN392648
SI  - GENBANK/FN392649
SI  - GENBANK/FN392650
SI  - GENBANK/FN392651
SI  - GENBANK/FN392652
SI  - GENBANK/FN392653
SI  - GENBANK/FN392654
SI  - GENBANK/FN392655
SI  - GENBANK/FN392656
SI  - GENBANK/FN392657
SI  - GENBANK/FN392658
SI  - GENBANK/FN392659
SI  - GENBANK/FN392660
SI  - GENBANK/FN392661
SI  - GENBANK/FN392662
SI  - GENBANK/FN392663
SI  - GENBANK/FN392664
SI  - GENBANK/FN392665
SI  - GENBANK/FN392666
SI  - GENBANK/FN392667
SI  - GENBANK/FN392668
SI  - GENBANK/FN392669
SI  - GENBANK/FN392670
SI  - GENBANK/FN392671
SI  - GENBANK/FN392672
SI  - GENBANK/FN392673
SI  - GENBANK/FN392674
SI  - GENBANK/FN392675
SI  - GENBANK/FN392676
SI  - GENBANK/FN392677
SI  - GENBANK/FN392678
SI  - GENBANK/FN392679
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100320
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (Arsenates)
RN  - 0 (Arsenites)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil Pollutants)
RN  - N712M78A8G (Arsenic)
SB  - IM
MH  - Arsenates/metabolism
MH  - Arsenic/*toxicity
MH  - Arsenites/metabolism
MH  - Bacteria/*classification/*drug effects/growth & development/isolation & 
      purification
MH  - Bacterial Proteins/genetics
MH  - Cirsium/*growth & development/*microbiology
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - *Drug Resistance, Bacterial
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - Plant Roots/microbiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Soil Pollutants/*toxicity
EDAT- 2010/03/23 06:00
MHDA- 2010/06/16 06:00
CRDT- 2010/03/23 06:00
PHST- 2009/10/30 00:00 [received]
PHST- 2010/01/25 00:00 [revised]
PHST- 2010/02/01 00:00 [accepted]
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
AID - S0723-2020(10)00028-7 [pii]
AID - 10.1016/j.syapm.2010.02.004 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2010 Apr;33(3):154-64. doi: 10.1016/j.syapm.2010.02.004. 
      Epub 2010 Mar 20.