PMID- 20304168
OWN - NLM
STAT- MEDLINE
DCOM- 20100819
LR  - 20161125
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 42
IP  - 2
DP  - 2010 Mar
TI  - Quantitative analyses of kidney injury molecule-1 messenger RNA in kidney 
      transplant recipients with graft dysfunction.
PG  - 473-4
LID - 10.1016/j.transproceed.2010.01.042 [doi]
AB  - BACKGROUND: Kidney injury molecule-1 (KIM-1), a type I transmembrane protein that 
      is not expressed in normal renal tissue, shows increased expression in 
      dedifferentiated cells within damaged regions of the proximal tubule. We 
      evaluated mRNA transcription of the KIM-1 gene in renal tissue of kidney 
      transplant patients who were experiencing graft dysfunction searching for an 
      accurate biomarker of kidney graft injury. PATIENTS AND METHODS: mRNA analysis 
      was performed on 59 biopsies from kidney transplant patients who had been 
      classified according to the Banff 1997 scheme. Biopsies were categorized in 5 
      diagnostic groups: acute tubular necrosis (ATN) with superimposed acute rejection 
      episode (ARE), ATN; ARE; calcineurin inhibitor nephrotoxicity (CIN); or 
      interstitial fibrosis and tubular atrophy (IFTA). Amplified tissue RNA was 
      quantified by real-time polymerase chain reaction. RESULTS: Renal tissue 
      evaluations showed significantly increased KIM-1 mRNA expression as shown by 
      median values, 25-75 percentiles, and averages of the logarithmic transformation: 
      namely, CIN group (50.6; 1.8-285, 1; 1.24) and IFTA group (7.5; 1.26-14.6; 0.62), 
      displayed significant differences (P > .05). In contrast, expression was lower 
      among the ATN (0.47; 0.28-1.06; -0.13); ARE (0.21; 0.11-0.78; -0.45), and ATN+ARE 
      (0.46; 0.06-3.27; -0.25) cohorts. CONCLUSION: These preliminary data suggested 
      that KIM-1 mRNA might be useful biomarker of tubular damage associated with CIN 
      and IFTA.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Nogare, A L
AU  - Nogare AL
AD  - Division of Nephrology, Hospital de Clinicas de Porto Alegre and Post-Graduate 
      Medical Sciences Course, School of Medicine, Federal University of Rio Grande do 
      Sul, Ramiro Barcelos Avenue, Porto Alegre, RS 90035-903. Brazil
FAU - Joelsons, G
AU  - Joelsons G
FAU - Pedroso, J A R
AU  - Pedroso JA
FAU - Veronese, F J V
AU  - Veronese FJ
FAU - Goncalves, L F
AU  - Goncalves LF
FAU - Manfro, R C
AU  - Manfro RC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (HAVCR1 protein, human)
RN  - 0 (Hepatitis A Virus Cellular Receptor 1)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Virus)
SB  - IM
MH  - Atrophy
MH  - Biopsy
MH  - Gene Expression Regulation
MH  - Hepatitis A Virus Cellular Receptor 1
MH  - Humans
MH  - Kidney Transplantation/*pathology
MH  - Kidney Tubules/pathology
MH  - Membrane Glycoproteins/*genetics
MH  - Necrosis
MH  - Postoperative Complications/epidemiology
MH  - RNA, Messenger/*genetics
MH  - Receptors, Virus/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2010/03/23 06:00
MHDA- 2010/08/20 06:00
CRDT- 2010/03/23 06:00
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/08/20 06:00 [medline]
AID - S0041-1345(10)00133-8 [pii]
AID - 10.1016/j.transproceed.2010.01.042 [doi]
PST - ppublish
SO  - Transplant Proc. 2010 Mar;42(2):473-4. doi: 10.1016/j.transproceed.2010.01.042.