PMID- 20304455
OWN - NLM
STAT- MEDLINE
DCOM- 20100510
LR  - 20100430
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 401
IP  - 2
DP  - 2010 Jun 5
TI  - Complete genomic sequence and an infectious BAC clone of feline herpesvirus-1 
      (FHV-1).
PG  - 215-27
LID - 10.1016/j.virol.2010.02.021 [doi]
AB  - Infection with feline herpesvirus-1 (FHV-1) is a major cause of upper respiratory 
      and ocular diseases in Felidae. We report the first complete genomic sequence of 
      FHV-1, as well as the construction and characterization of a bacterial artificial 
      chromosome (BAC) clone of FHV-1, which contains the entire FHV-1 genome and has 
      the BAC vector inserted at the left end of U(L). Complete genomic sequences were 
      derived from both the FHV-1 BAC clone and purified virion DNA. The FHV-1 genome 
      is 135,797bp in size with an overall G+C content of 45%. A total of 78 open 
      reading frames were predicted, encoding 74 distinct proteins. The gene 
      arrangement is collinear with that of most sequenced varicelloviruses. The virus 
      regenerated from the BAC was very similar to the parental C-27 strain in vitro in 
      terms of plaque morphology and growth characteristics and highly virulent in cats 
      in a preliminary in vivo study.
CI  - 2010 Elsevier Inc. All rights reserved.
FAU - Tai, S H Sheldon
AU  - Tai SH
AD  - Graduate Program in Comparative Medicine and Integrative Biology, College of 
      Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
FAU - Niikura, Masahiro
AU  - Niikura M
FAU - Cheng, Hans H
AU  - Cheng HH
FAU - Kruger, John M
AU  - Kruger JM
FAU - Wise, Annabel G
AU  - Wise AG
FAU - Maes, Roger K
AU  - Maes RK
LA  - eng
SI  - GENBANK/FJ478159
SI  - GENBANK/GU250525
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100321
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Composition
MH  - Cat Diseases/pathology/virology
MH  - Cats
MH  - *Chromosomes, Artificial, Bacterial
MH  - DNA, Viral/chemistry/*genetics
MH  - Felidae/*virology
MH  - Gene Order
MH  - *Genome, Viral
MH  - Herpesviridae/*genetics/isolation & purification/*pathogenicity
MH  - Herpesviridae Infections/pathology/*veterinary
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Sequence Analysis, DNA
MH  - Synteny
EDAT- 2010/03/23 06:00
MHDA- 2010/05/11 06:00
CRDT- 2010/03/23 06:00
PHST- 2009/11/26 00:00 [received]
PHST- 2009/12/22 00:00 [revised]
PHST- 2010/02/17 00:00 [accepted]
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/05/11 06:00 [medline]
AID - S0042-6822(10)00130-3 [pii]
AID - 10.1016/j.virol.2010.02.021 [doi]
PST - ppublish
SO  - Virology. 2010 Jun 5;401(2):215-27. doi: 10.1016/j.virol.2010.02.021. Epub 2010 
      Mar 21.