PMID- 20304916
OWN - NLM
STAT- MEDLINE
DCOM- 20100721
LR  - 20220224
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 285
IP  - 25
DP  - 2010 Jun 18
TI  - Distribution and function of macrophage galactose-type C-type lectin 2 
      (MGL2/CD301b): efficient uptake and presentation of glycosylated antigens by 
      dendritic cells.
PG  - 19193-204
LID - 10.1074/jbc.M110.113613 [doi]
AB  - Dendritic cells (DCs) express cell surface lectins that are potentially involved 
      in the recognition, uptake, and presentation of glycosylated foreign substances. 
      A unique calcium-type (C-type) lectin, the macrophage galactose (Gal)-type C-type 
      lectin (MGL/CD301) expressed on DCs, is thought to participate in the recognition 
      of molecules from both altered self and pathogens due to its monosaccharide 
      specificity for Gal and N-acetylgalactosamine (GalNAc). Although mice have two 
      MGL genes, Mgl1 and Mgl2, their distinct roles have not been previously explored. 
      The present report characterizes the properties of MGL2 by examining its 
      distribution and its role in antigen presentation by DCs. We generated an 
      MGL2-specific monoclonal antibody and examined MGL2 expression in tissues by 
      immunohistochemistry and in isolated cells by flow cytometry. The cells reactive 
      with this antibody were shown to be a portion of MGL1-expressing cells, mostly 
      conventional DCs. Internalization of soluble polyacrylamide polymers (PAA) with 
      alpha-GalNAc residues (GalNAc-PAA) by bone marrow-derived DCs (BM-DCs) was 
      mediated by MGL2, as revealed by a comparison of Mgl1(-/-) and Mgl2(-/-) BM-DCs 
      with wild-type BM-DCs. Biotinylated GalNAc-PAA conjugated to streptavidin (SAv) 
      was more efficiently presented to SAv-primed T cells by BM-DCs than 
      beta-N-acetylglucosamine-PAA conjugated to SAv or SAv alone as shown by thymidine 
      uptake and cytokine production. This is the first report that demonstrates the 
      involvement of GalNAc residues in antigen uptake and presentation by DCs that 
      lead to CD4(+) T cell activation.
FAU - Denda-Nagai, Kaori
AU  - Denda-Nagai K
AD  - Laboratory of Cancer Biology and Molecular Immunology, Graduate School of 
      Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
FAU - Aida, Satoshi
AU  - Aida S
FAU - Saba, Kengo
AU  - Saba K
FAU - Suzuki, Kiwamu
AU  - Suzuki K
FAU - Moriyama, Saya
AU  - Moriyama S
FAU - Oo-Puthinan, Sarawut
AU  - Oo-Puthinan S
FAU - Tsuiji, Makoto
AU  - Tsuiji M
FAU - Morikawa, Akiko
AU  - Morikawa A
FAU - Kumamoto, Yosuke
AU  - Kumamoto Y
FAU - Sugiura, Daisuke
AU  - Sugiura D
FAU - Kudo, Akihiko
AU  - Kudo A
FAU - Akimoto, Yoshihiro
AU  - Akimoto Y
FAU - Kawakami, Hayato
AU  - Kawakami H
FAU - Bovin, Nicolai V
AU  - Bovin NV
FAU - Irimura, Tatsuro
AU  - Irimura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100319
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens)
RN  - 0 (Lectins, C-Type)
RN  - 0 (MGL2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antigens/chemistry
MH  - Bone Marrow Cells/cytology
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - Dendritic Cells/*cytology
MH  - Female
MH  - Flow Cytometry/methods
MH  - Glycosylation
MH  - Immunohistochemistry/methods
MH  - Lectins, C-Type/*metabolism
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes/metabolism
MH  - Tissue Distribution
PMC - PMC2885198
EDAT- 2010/03/23 06:00
MHDA- 2010/07/22 06:00
PMCR- 2011/06/18
CRDT- 2010/03/23 06:00
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/07/22 06:00 [medline]
PHST- 2011/06/18 00:00 [pmc-release]
AID - S0021-9258(20)58051-4 [pii]
AID - M110.113613 [pii]
AID - 10.1074/jbc.M110.113613 [doi]
PST - ppublish
SO  - J Biol Chem. 2010 Jun 18;285(25):19193-204. doi: 10.1074/jbc.M110.113613. Epub 
      2010 Mar 19.