PMID- 20304952
OWN - NLM
STAT- MEDLINE
DCOM- 20101215
LR  - 20240511
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Print)
IS  - 0002-9440 (Linking)
VI  - 176
IP  - 5
DP  - 2010 May
TI  - Dendritic cells produce CXCL13 and participate in the development of murine small 
      intestine lymphoid tissues.
PG  - 2367-77
LID - 10.2353/ajpath.2010.090723 [doi]
AB  - In the adult intestine, luminal microbiota induce cryptopatches to transform into 
      isolated lymphoid follicles (ILFs), which subsequently act as sites for the 
      generation of IgA responses. The events leading to this conversion are 
      incompletely understood. Dendritic cells (DCs) are components of cryptopatches 
      (CPs) and ILFs and were therefore evaluated in this process. We observed that the 
      adult murine intestine contains clusters of DCs restricted to the CP/ILF 
      continuum. A numerical and cell associative hierarchy in the adult intestine and 
      a chronologic hierarchy in the neonatal intestine demonstrated that these 
      clusters form after the coalescence of CD90+ cells to form CPs and before the 
      influx of B220+ B lymphocytes to form ILFs. Cluster formation was dependent on 
      lymphotoxin and the lymphotoxin beta receptor and independent of lymphocytes. The 
      ILF DC population was distinguished from that of the lamina propria by the 
      absence of CD4+CD11c+ cells and an increased proportion of CD11c+B220+ cells. The 
      formation of clusters was not limited by DC numbers but was induced by luminal 
      microbiota. Moreover, in the absence of the chemokine CXCL13, CP transformation 
      into ILF was arrested. Furthermore, ILF DCs express CXCL13, and depletion of DCs 
      resulted in regression of ILFs and disorganization of CPs. These results reveal 
      DC participation in ILF transformation and maintenance and suggest that in part 
      this may be due to CXCL13 production by these cells.
FAU - McDonald, Keely G
AU  - McDonald KG
AD  - Department of Internal Medicine, Washington University School of Medicine, St. 
      Louis, Missouri, USA.
FAU - McDonough, Jacquelyn S
AU  - McDonough JS
FAU - Dieckgraefe, Brian K
AU  - Dieckgraefe BK
FAU - Newberry, Rodney D
AU  - Newberry RD
LA  - eng
GR  - R01 DK064798/DK/NIDDK NIH HHS/United States
GR  - P30 CA91842/CA/NCI NIH HHS/United States
GR  - P30 CA091842/CA/NCI NIH HHS/United States
GR  - AG028309/AG/NIA NIH HHS/United States
GR  - P30 DK052574/DK/NIDDK NIH HHS/United States
GR  - R01 DK064798-07/DK/NIDDK NIH HHS/United States
GR  - R21 AG028309/AG/NIA NIH HHS/United States
GR  - DK64798/DK/NIDDK NIH HHS/United States
GR  - P30-DK52574/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100319
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Chemokine CXCL13)
RN  - 0 (Cxcl13 protein, mouse)
RN  - 0 (Thy-1 Antigens)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/cytology
MH  - Chemokine CXCL13/*metabolism
MH  - Dendritic Cells/*cytology
MH  - Flow Cytometry/methods
MH  - *Gene Expression Regulation
MH  - Immune System
MH  - Intestine, Small/*metabolism
MH  - Leukocyte Common Antigens/metabolism
MH  - Lymphocytes/cytology
MH  - Lymphoid Tissue/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mucous Membrane/pathology
MH  - Thy-1 Antigens/biosynthesis
PMC - PMC2861101
EDAT- 2010/03/23 06:00
MHDA- 2010/12/16 06:00
PMCR- 2011/05/01
CRDT- 2010/03/23 06:00
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - S0002-9440(10)60032-6 [pii]
AID - 10.2353/ajpath.2010.090723 [doi]
PST - ppublish
SO  - Am J Pathol. 2010 May;176(5):2367-77. doi: 10.2353/ajpath.2010.090723. Epub 2010 
      Mar 19.