PMID- 20306103
OWN - NLM
STAT- MEDLINE
DCOM- 20100813
LR  - 20211020
IS  - 1435-604X (Electronic)
IS  - 0268-8921 (Linking)
VI  - 25
IP  - 4
DP  - 2010 Jul
TI  - Elevation of plasma membrane permeability upon laser irradiation of extracellular 
      microbubbles.
PG  - 587-94
LID - 10.1007/s10103-010-0773-1 [doi]
AB  - Laser-mediated gene transfection has received much attention as a new method for 
      targeted gene therapy because of the high controllability of laser energy and 
      direction. In this report, we describe a combination laser-microbubble system 
      that enables membrane-impermeable molecules to penetrate cell membranes. The main 
      theories we apply are optical breakdown and photoacoustic generation, which are 
      induced by laser irradiation. Firstly, different types of laser light (Ar-green, 
      Novus Varia poly-wavelength and Nd:YAG laser) were adopted to blast liposome 
      microbubble contrast medium; subsequently, the Nd:YAG laser (1064 nm, 4 ns), 
      which could successfully blast microbubbles, and ultrasound were used in 
      combination to irradiate a mixture of liposome microbubbles and retinoblastoma 
      (Rb) cells. After irradiation, membrane permeability was evaluated by flow 
      cytometric assay using propidium iodide (PI) and fluorescein diacetate (FDA). The 
      proportion of permeabilized resealed cells was affected by changes in the light 
      energy. All of the Nd:YAG laser, Nd:YAG combination laser-microbubble and 
      combination ultrasound-microbubble systems were able to permeabilize the Rb 
      cells. These results suggest that this combination laser-microbubble system is a 
      new means of delivering exogenous materials into living cells.
FAU - Zhou, Yu
AU  - Zhou Y
AD  - Departments of Ophthalmology, The Second Affiliated Hospital of Chongqing Medical 
      University, 74# Linjiang Road, Chongqing, 400010, People's Republic of China.
FAU - Zhou, Xi-Yuan
AU  - Zhou XY
FAU - Wang, Zhi-Gang
AU  - Wang ZG
FAU - Zhu, Ye-Feng
AU  - Zhu YF
FAU - Li, Pan
AU  - Li P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100320
PL  - England
TA  - Lasers Med Sci
JT  - Lasers in medical science
JID - 8611515
RN  - 0 (Fluoresceins)
RN  - 0 (Liposomes)
RN  - 36015-30-2 (Propidium)
RN  - YL39R93PRE (diacetylfluorescein)
SB  - IM
MH  - Cell Membrane/*radiation effects
MH  - Cell Membrane Permeability/*radiation effects
MH  - Fluoresceins/pharmacology
MH  - Gene Transfer Techniques
MH  - Humans
MH  - *Lasers
MH  - Liposomes
MH  - *Microbubbles
MH  - Microscopy, Fluorescence
MH  - Propidium/pharmacology
MH  - Retinoblastoma/pathology
MH  - Tumor Cells, Cultured
EDAT- 2010/03/23 06:00
MHDA- 2010/08/14 06:00
CRDT- 2010/03/23 06:00
PHST- 2009/11/16 00:00 [received]
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/08/14 06:00 [medline]
AID - 10.1007/s10103-010-0773-1 [doi]
PST - ppublish
SO  - Lasers Med Sci. 2010 Jul;25(4):587-94. doi: 10.1007/s10103-010-0773-1. Epub 2010 
      Mar 20.