PMID- 20306126
OWN - NLM
STAT- MEDLINE
DCOM- 20100920
LR  - 20211020
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 18
IP  - 3
DP  - 2010 Apr
TI  - Genome organization of major tandem repeats in the hard tick, Ixodes scapularis.
PG  - 357-70
LID - 10.1007/s10577-010-9120-4 [doi]
AB  - In spite of the global medical and veterinary importance of Ixodid ticks, 
      relatively little is known about their genome organization. To address this, we 
      developed the first fluorescence in situ hybridization (FISH)-based chromosome 
      markers in the Lyme disease vector, Ixodes scapularis. Shotgun genomic DNA (gDNA) 
      sequences were used to identify three major tandem repeat families which were 
      localized to specific heterochromatic regions of I. scapularis chromosomes 
      prepared from the mitotic cell line ISE18. Together, these repeats were estimated 
      to contribute approximately 159 Mb (8%) of the 2.1 Gb (haploid) I. scapularis 
      genome. The relative arrangement of each tandem repeat family and the nucleolar 
      organizing regions was determined by rehybridization to individual chromosome 
      spreads, which was useful to distinguish different chromosomes in the ISE18 
      karyotype. Long stretches (>20 kb) of tandem repeat-containing gDNA were 
      resistant to digestion by the methylation-sensitive restriction enzyme HpaII and 
      localized to the presumed peri-centromeric regions of the chromosomes. A 
      telomeric probe based on the arthropod-conserved (TTAGG)(n) tandemly repetitive 
      motif was localized to the termini of each I. scapularis chromosome. Localization 
      of these markers produced the first link between DNA sequences and major 
      structural features of I. scapularis chromosomes and thereby provided the 
      framework for a FISH-based physical map.
FAU - Meyer, Jason M
AU  - Meyer JM
AD  - Department of Entomology, Purdue University, West Lafayette, IN 47907-2089, USA. 
      meyerjm@purdue.edu
FAU - Kurtti, Timothy J
AU  - Kurtti TJ
FAU - Van Zee, Janice P
AU  - Van Zee JP
FAU - Hill, Catherine A
AU  - Hill CA
LA  - eng
GR  - HHS2662004000396/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100320
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Centromere/genetics
MH  - Computational Biology
MH  - Consensus Sequence
MH  - Cytogenetic Analysis
MH  - DNA/genetics
MH  - DNA Probes/metabolism
MH  - Genome/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Ixodes/*genetics
MH  - Molecular Sequence Data
MH  - Software
MH  - Tandem Repeat Sequences/*genetics
MH  - Telomere/genetics
EDAT- 2010/03/23 06:00
MHDA- 2010/09/21 06:00
CRDT- 2010/03/23 06:00
PHST- 2010/01/15 00:00 [received]
PHST- 2010/02/09 00:00 [accepted]
PHST- 2010/03/23 06:00 [entrez]
PHST- 2010/03/23 06:00 [pubmed]
PHST- 2010/09/21 06:00 [medline]
AID - 10.1007/s10577-010-9120-4 [doi]
PST - ppublish
SO  - Chromosome Res. 2010 Apr;18(3):357-70. doi: 10.1007/s10577-010-9120-4. Epub 2010 
      Mar 20.