PMID- 20307499
OWN - NLM
STAT- MEDLINE
DCOM- 20100513
LR  - 20220321
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 394
IP  - 4
DP  - 2010 Apr 16
TI  - Calcium-sensing receptor activation contributed to apoptosis stimulates TRPC6 
      channel in rat neonatal ventricular myocytes.
PG  - 955-61
LID - 10.1016/j.bbrc.2010.03.096 [doi]
AB  - Capacitative calcium entry (CCE) refers to the influx of calcium through plasma 
      membrane channels activated on depletion of endoplasmic sarcoplasmic/reticulum 
      (ER/SR) Ca(2+) stores, which is performed mainly by the transient receptor 
      potential (TRP) channels. TRP channels are expressed in cardiomyocytes. 
      Calcium-sensing receptor (CaR) is also expressed in rat cardiac tissue and plays 
      an important role in mediating cardiomyocyte apoptosis. However, there are no 
      data regarding the link between CaR and TRP channels in rat heart. In this study, 
      in rat neonatal myocytes, by Ca(2+) imaging, we found that the depletion of ER/SR 
      Ca(2+) stores by thapsigargin (TG) elicited a transient rise in cytoplasmic 
      Ca(2+) ([Ca(2+)](i)), followed by sustained increase depending on extracellular 
      Ca(2+). But, TRP channels inhibitor (SKF96365), not L-type channels or the 
      Na(+)/Ca(2+) exchanger inhibitors, inhibited [Ca(2+)](i) relatively high. Then, 
      we found that the stimulation of CaR with its activator gadolinium chloride 
      (GdCl(3)) or by an increased extracellular Ca(2+)([Ca(2+)](o)) increased the 
      concentration of intracelluar Ca(2+), whereas, the sustained elevation of 
      [Ca(2+)](i) was reduced in the presence of SKF96365. Similarly, the duration of 
      [Ca(2+)](i) increase was also shortened in the absence of extracellular Ca(2+). 
      Western blot analysis showed that GdCl(3) increased the expression of TRPC6, 
      which was reversed by SKF96365. Additionally, SKF96365 reduced cardiomyocyte 
      apoptosis induced by GdCl(3). Our results suggested that CCE exhibited in rat 
      neonatal myocytes and CaR activation induced Ca(2+)-permeable cationic channels 
      TRPCs to gate the CCE, for which TRPC6 was one of the most likely candidates. 
      TRPC6 channel was functionally coupled with CaR to enhance the cardiomyocyte 
      apoptosis.
CI  - 2010 Elsevier Inc. All rights reserved.
FAU - Sun, Yi-hua
AU  - Sun YH
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital of Harbin 
      Medical University, Harbin 150086, China.
FAU - Li, Yong-quan
AU  - Li YQ
FAU - Feng, Shan-li
AU  - Feng SL
FAU - Li, Bao-xin
AU  - Li BX
FAU - Pan, Zhen-wei
AU  - Pan ZW
FAU - Xu, Chang-qing
AU  - Xu CQ
FAU - Li, Ting-ting
AU  - Li TT
FAU - Yang, Bao-feng
AU  - Yang BF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100320
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Imidazoles)
RN  - 0 (Receptors, Calcium-Sensing)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (Trpc6 protein, rat)
RN  - 67526-95-8 (Thapsigargin)
RN  - AU0V1LM3JT (Gadolinium)
RN  - I61V87164A (1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole)
RN  - P7082WY76D (gadolinium chloride)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Calcium/metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Gadolinium/pharmacology
MH  - Heart Ventricles/cytology/*metabolism
MH  - Imidazoles/pharmacology
MH  - Muscle Cells/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Calcium-Sensing/*agonists
MH  - TRPC Cation Channels/*agonists
MH  - Thapsigargin/pharmacology
EDAT- 2010/03/24 06:00
MHDA- 2010/05/14 06:00
CRDT- 2010/03/24 06:00
PHST- 2010/03/02 00:00 [received]
PHST- 2010/03/16 00:00 [accepted]
PHST- 2010/03/24 06:00 [entrez]
PHST- 2010/03/24 06:00 [pubmed]
PHST- 2010/05/14 06:00 [medline]
AID - S0006-291X(10)00560-7 [pii]
AID - 10.1016/j.bbrc.2010.03.096 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Apr 16;394(4):955-61. doi: 
      10.1016/j.bbrc.2010.03.096. Epub 2010 Mar 20.