PMID- 20307513
OWN - NLM
STAT- MEDLINE
DCOM- 20100810
LR  - 20100507
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1332
DP  - 2010 May 21
TI  - Bone marrow stromal cell transplantation for treatment of sub-acute spinal cord 
      injury in the rat.
PG  - 32-47
LID - 10.1016/j.brainres.2010.03.043 [doi]
AB  - Bone marrow stromal cells (BMSCs) have been studied as effective transplants for 
      the treatment of spinal cord injury (SCI). Our previous study showed that BMSCs 
      infused into the cerebrospinal fluid (CSF) exhibited distinct effects on the 
      recovery of acute SCI. The present study examined the effects of BMSCs in 
      sub-acute SCI (2weeks post-injury) by transplanting them directly into the 
      lesion. The spinal cord was crush-injured at the Th8-9 level in rats, and 2weeks 
      later, cultured BMSCs (5x10(5)) derived from GFP-transgenic rats of the same 
      strain were transplanted into the lesion. Tissue repair and nerve regeneration 
      were examined by immunohistochemistry and electron microscopy. GFP-labeled BMSCs 
      survived as cell assemblies in the spinal cord for 1-2weeks after 
      transplantation. The dorsal side of BMSC assemblies in the spinal cord usually 
      showed an expanded GFAP-negative, astrocyte-devoid area, in which extracellular 
      matrices including collagen fibrils were deposited. Numerous regenerating axons 
      associated with Schwann cells grew out through such astrocyte-devoid 
      extracellular matrices. Ascending (CGRP-containing) and descending (5HT- and 
      TH-containing) axons were included in these regenerating axons. Regenerated axons 
      were myelinated by Schwann cells beyond 2weeks post-transplantation. Cavity 
      formation was reduced in the cell transplantation group. Locomotory behavior 
      assessed by the BBB scale improved to 9.8 points in the cell transplantation 
      group, while it was to 5.5-5.7 in the control. BMSC transplantation into lesions 
      of advanced SCI has markedly beneficial effects on tissue repair and axonal 
      outgrowth, leading to improved locomotion in rats.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Ide, Chizuka
AU  - Ide C
AD  - Department of Occupational Therapy, Faculty of Nursing and Rehabilitation, Aino 
      University, Higashi-ohda, 4-5-4, Ibaragi City, Osaka, Japan. 
      c-ide@ot-u.aino.ac.jp
FAU - Nakai, Yoshiyasu
AU  - Nakai Y
FAU - Nakano, Norihiko
AU  - Nakano N
FAU - Seo, Tae-Beom
AU  - Seo TB
FAU - Yamada, Yoshihiro
AU  - Yamada Y
FAU - Endo, Katsuaki
AU  - Endo K
FAU - Noda, Toru
AU  - Noda T
FAU - Saito, Fukuki
AU  - Saito F
FAU - Suzuki, Yoshihisa
AU  - Suzuki Y
FAU - Fukushima, Masanori
AU  - Fukushima M
FAU - Nakatani, Toshio
AU  - Nakatani T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100319
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Astrocytes/physiology
MH  - Axons/physiology
MH  - Bone Marrow Cells/physiology
MH  - Bone Marrow Transplantation/*methods
MH  - Cells, Cultured
MH  - Collagen/metabolism
MH  - Disease Models, Animal
MH  - Extracellular Matrix/physiology
MH  - Female
MH  - Green Fluorescent Proteins/genetics
MH  - Locomotion/physiology
MH  - Myelin Sheath/physiology
MH  - Nerve Regeneration/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Transgenic
MH  - Schwann Cells/physiology
MH  - Spinal Cord Injuries/pathology/physiopathology/*surgery
MH  - Stromal Cells/cytology/physiology/*transplantation
MH  - Time Factors
EDAT- 2010/03/24 06:00
MHDA- 2010/08/11 06:00
CRDT- 2010/03/24 06:00
PHST- 2009/10/02 00:00 [received]
PHST- 2010/03/11 00:00 [revised]
PHST- 2010/03/15 00:00 [accepted]
PHST- 2010/03/24 06:00 [entrez]
PHST- 2010/03/24 06:00 [pubmed]
PHST- 2010/08/11 06:00 [medline]
AID - S0006-8993(10)00616-5 [pii]
AID - 10.1016/j.brainres.2010.03.043 [doi]
PST - ppublish
SO  - Brain Res. 2010 May 21;1332:32-47. doi: 10.1016/j.brainres.2010.03.043. Epub 2010 
      Mar 19.