PMID- 20331449
OWN - NLM
STAT- MEDLINE
DCOM- 20110308
LR  - 20100921
IS  - 1365-2133 (Electronic)
IS  - 0007-0963 (Linking)
VI  - 163
IP  - 4
DP  - 2010 Oct
TI  - A clinical isolate of Leishmania donovani with ITS1 sequence polymorphism as a 
      cause of para-kala-azar dermal leishmaniasis in an Ethiopian human 
      immunodeficiency virus-positive patient on highly active antiretroviral therapy.
PG  - 870-4
LID - 10.1111/j.1365-2133.2010.09768.x [doi]
AB  - The diagnosis of para-kala-azar dermal leishmaniasis (PKDL/VL), either as an 
      immune reconstitution inflammatory syndrome (IRIS)-associated syndrome or as a 
      complication of visceral leishmaniasis (VL) during human immunodeficiency virus 
      (HIV) co-infection in patients with or without highly active antiretroviral 
      therapy, represents a challenge for prompt treatment. The aim of this study was 
      to identify the causative Leishmania species and to clarify whether the 
      post-kala-azar dermal leishmaniasis (PKDL)-like lesions appeared as a result of 
      IRIS or not. A 31-year-old Ethiopian male patient, with stage IV HIV/acquired 
      immune deficiency syndrome (AIDS), was clinically diagnosed with PKDL/VL. He had 
      developed a generalized maculopapular rash on his face after initiation of highly 
      active antiretroviral therapy. The Leishmania isolate obtained from the skin 
      lesions was analysed by polymerase chain reaction-restriction fragment length 
      polymorphism (PCR-RFLP) and sequencing of the ribosomal DNA internal transcribed 
      spacer 1 (ITS1) and partial coding sequences of the heat shock protein 70 gene 
      (hsp70). Restriction analysis of the ITS1 PCR product gave a unique RFLP pattern 
      not seen before for any Leishmania isolate. Sequencing of both the ITS1 and hsp70 
      PCR products identified the causative species as Leishmania donovani, and further 
      revealed the existence of five different sequence variants of the ITS1 among the 
      10 clones sequenced. The results indicate that PKDL/VL resulted from an infection 
      by L. donovani. The sequence variants of ITS1 might be due to the presence of 
      multiple strains/clones or the existence of intragenomic variations in the 
      multicopy ITS1, or a combination of both.
CI  - (c) 2010 The Authors. Journal Compilation (c) 2010 British Association of 
      Dermatologists.
FAU - Gelanew, T
AU  - Gelanew T
AD  - Institut fur Mikrobiologie und Hygiene, Charite Universitatsmedizin Berlin, 
      Dorotheenstrasse 96, D-10117 Berlin, Germany. tesfayegela2002@yahoo.com
FAU - Amogne, W
AU  - Amogne W
FAU - Abebe, T
AU  - Abebe T
FAU - Kuhls, K
AU  - Kuhls K
FAU - Hailu, A
AU  - Hailu A
FAU - Schonian, G
AU  - Schonian G
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Dermatol
JT  - The British journal of dermatology
JID - 0004041
RN  - 0 (DNA, Protozoan)
RN  - 0 (DNA, Ribosomal Spacer)
SB  - IM
MH  - Adult
MH  - Antiretroviral Therapy, Highly Active
MH  - Base Sequence
MH  - DNA, Protozoan/genetics
MH  - DNA, Ribosomal Spacer/*genetics
MH  - HIV Infections/*complications/drug therapy
MH  - Humans
MH  - Leishmania donovani/genetics/*isolation & purification
MH  - Leishmaniasis, Visceral/*complications/parasitology
MH  - Male
MH  - Molecular Sequence Data
MH  - Sequence Alignment
EDAT- 2010/03/25 06:00
MHDA- 2011/03/09 06:00
CRDT- 2010/03/25 06:00
PHST- 2010/03/25 06:00 [entrez]
PHST- 2010/03/25 06:00 [pubmed]
PHST- 2011/03/09 06:00 [medline]
AID - BJD9768 [pii]
AID - 10.1111/j.1365-2133.2010.09768.x [doi]
PST - ppublish
SO  - Br J Dermatol. 2010 Oct;163(4):870-4. doi: 10.1111/j.1365-2133.2010.09768.x.