PMID- 20334160
OWN - NLM
STAT- MEDLINE
DCOM- 20100503
LR  - 20191210
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 93
IP  - 1
DP  - 2010 Jan-Feb
TI  - Evaluation of immunoassays as an alternative for the rapid determination of 
      pesticides in wine and grape samples.
PG  - 2-11
AB  - The main objective of this paper is to address the performance of immunochemical 
      assays for the detection of the residues of three pesticides [atrazine, 
      bromopropylate, and 2,4,6-trichlorophenol (TCP)] in real winery samples, such as 
      wine, grapes, and grape juice. Different approaches have been evaluated to 
      minimize interferences from the matrixes, and suitable working protocols have 
      been established in order to achieve the necessary LODs, accuracy, and precision 
      for real samples. A simple dilution of the sample proved to be sufficient for the 
      determination of atrazine and bromopropylate in red and white wine and grape 
      juice at the required levels of concentration. However, for TCP, an SPE procedure 
      has been optimized using amino cartridges. The recoveries were above 85% in all 
      cases, and the LOD values were below the parts per billion level, except for 
      bromopropylate, which ranged between 2 and 50 microg/L, depending on the matrix. 
      The grape matrix effect could be resolved by a simple extraction with methanol. 
      Complete recoveries were obtained, and the final measurement procedures were able 
      to determine selected pesticides below their maximum residue levels. The newly 
      developed methods have been compared with standard chromatographic methods.
FAU - Argarate, Nerea
AU  - Argarate N
AD  - AZTI-Tecnalia, Food Research Division, 48160 Derio, Bizkaia, Spain.
FAU - Arestin, Maria
AU  - Arestin M
FAU - Ramon-Azcon, Javier
AU  - Ramon-Azcon J
FAU - Alfaro, Begona
AU  - Alfaro B
FAU - Barranco, Alejandro
AU  - Barranco A
FAU - Sanchez-Baeza, Francisco
AU  - Sanchez-Baeza F
FAU - Marco, M Pilar
AU  - Marco MP
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Benzilates)
RN  - 0 (Chlorophenols)
RN  - 0 (Pesticides)
RN  - 3U468ZM257 (isopropyl 4,4'-dibromobenzilate)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
RN  - QJA9M5H4IM (Atrazine)
SB  - IM
MH  - Atrazine/analysis/toxicity
MH  - Benzilates/analysis/toxicity
MH  - Chlorophenols/analysis/toxicity
MH  - Chromatography, High Pressure Liquid
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Food Contamination/analysis
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunochemistry/methods
MH  - Pesticides/*analysis/toxicity
MH  - Vitis/*chemistry/toxicity
MH  - Wine/*analysis/toxicity
EDAT- 2010/03/26 06:00
MHDA- 2010/05/04 06:00
CRDT- 2010/03/26 06:00
PHST- 2010/03/26 06:00 [entrez]
PHST- 2010/03/26 06:00 [pubmed]
PHST- 2010/05/04 06:00 [medline]
PST - ppublish
SO  - J AOAC Int. 2010 Jan-Feb;93(1):2-11.