PMID- 20337937
OWN - NLM
STAT- MEDLINE
DCOM- 20110926
LR  - 20110615
IS  - 1439-0507 (Electronic)
IS  - 0933-7407 (Linking)
VI  - 54
IP  - 4
DP  - 2011 Jul
TI  - Antifungal activity of trichothecenes from Fusarium sp. against clinical isolates 
      of Paracoccidioides brasiliensis.
PG  - e122-9
LID - 10.1111/j.1439-0507.2009.01854.x [doi]
AB  - Paracoccidioidomycosis (PCM), a human mycosis caused by the dimorphic fungus 
      Paracoccidioides brasiliensis, is a serious public health problem in several 
      countries of Latin America. In our search we found that the crude extract of the 
      endophytic fungus UFMGCB 551 was able to inhibit several clinical strains of P. 
      brasiliensis, and was also active in the bioautographic assay against 
      Cladosporium sphaerospermum. The endophytic fungus UFMGCB 551 was isolated from 
      the plant Piptadenia adiantoides J.F. Macbr (Fabaceae). The fungus was identified 
      as Fusarium sp. based on its macro- and micro-morphology, and on the sequence of 
      the internally transcribed spacer regions (ITS) of its rRNA gene. The 
      chromatographic fractionation of the fungal extract was guided by the 
      bioautographic assay to afford three known trichothecene mycotoxins: T2-toxin (1) 
      and a mixture of 8-n-butyrylneosolaniol (2) and 8-isobutyrylsolaniol (3). The 
      minimal inhibitory concentrations (MIC) of the these compounds against eleven 
      clinical strains of P. brasiliensis were evaluated and found to be in the range 
      between 75 and 640 nmol l(-1) for 1 and 160-640 nmol l(-1) for the mixture of 2 
      and 3.
CI  - (c) 2010 Blackwell Verlag GmbH.
FAU - Campos, Fernanda Fraga
AU  - Campos FF
AD  - Laboratorio de Quimica de Produtos Naturais, Centro de Pesquisas Rene Rachou, 
      Belo Horizonte, Brazil. fcampos@cpqrr.fiocruz.br
FAU - Johann, Susana
AU  - Johann S
FAU - Cota, Betania Barros
AU  - Cota BB
FAU - Alves, Tania Maria Almeida
AU  - Alves TM
FAU - Rosa, Luiz Henrique
AU  - Rosa LH
FAU - Caligiorne, Rachel Basques
AU  - Caligiorne RB
FAU - Cisalpino, Patricia Silva
AU  - Cisalpino PS
FAU - Rosa, Carlos Augusto
AU  - Rosa CA
FAU - Zani, Carlos Leomar
AU  - Zani CL
LA  - eng
SI  - GENBANK/GQ325261
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100311
PL  - Germany
TA  - Mycoses
JT  - Mycoses
JID - 8805008
RN  - 0 (Antifungal Agents)
RN  - 0 (DNA, Fungal)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Ribosomal Spacer)
RN  - 0 (Trichothecenes)
SB  - IM
MH  - Antifungal Agents/chemistry/*isolation & purification/*pharmacology
MH  - Chromatography/methods
MH  - DNA, Fungal/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - DNA, Ribosomal Spacer/chemistry/genetics
MH  - Fabaceae/microbiology
MH  - Fusarium/*chemistry/classification/genetics/isolation & purification
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Molecular Sequence Data
MH  - Paracoccidioides/*drug effects/isolation & purification
MH  - Paracoccidioidomycosis/microbiology
MH  - Sequence Analysis, DNA
MH  - Trichothecenes/chemistry/*isolation & purification/*pharmacology
EDAT- 2010/03/27 06:00
MHDA- 2011/09/29 06:00
CRDT- 2010/03/27 06:00
PHST- 2010/03/27 06:00 [entrez]
PHST- 2010/03/27 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - MYC1854 [pii]
AID - 10.1111/j.1439-0507.2009.01854.x [doi]
PST - ppublish
SO  - Mycoses. 2011 Jul;54(4):e122-9. doi: 10.1111/j.1439-0507.2009.01854.x. Epub 2010 
      Mar 11.