PMID- 20340097
OWN - NLM
STAT- MEDLINE
DCOM- 20100525
LR  - 20100422
IS  - 1934-662X (Print)
IS  - 1934-662X (Linking)
VI  - 118
IP  - 2
DP  - 2010 Apr 25
TI  - The role of fluorescence in situ hybridization and polymerase chain reaction in 
      the diagnosis and classification of lymphoproliferative disorders on fine-needle 
      aspiration.
PG  - 105-12
LID - 10.1002/cncy.20070 [doi]
AB  - BACKGROUND: Fine-needle aspiration (FNA) has been used in the evaluation of 
      lymphadenopathy for a long time and is highly reliable in the identification of 
      metastatic malignancies. However, the role of FNA in the assessment of new 
      lymphoproliferative disorders continues to be a subject of debate. The objective 
      of the current study was to evaluate the role of molecular cytogenetic studies in 
      FNA diagnoses of lymphoproliferative disorders. METHODS: A retrospective, 
      computer-based search for lymph node FNAs from 2006 to 2007 was performed. Cases 
      with either fluorescence in situ hybridization (FISH) and/or polymerase chain 
      reaction (PCR) studies were subjected to further analysis. RESULTS: In total, 243 
      lymph node FNAs were performed during the period, including 104 that were 
      positive/suspicious for metastatic malignancies, 16 that were positive/suspicious 
      for lymphomas, 15 that demonstrated atypical lymphoid proliferation, 73 that were 
      reactive, 14 that were deemed granulomas, and 21 that were determined to be 
      nondiagnostic. Molecular analysis included combined FISH/PCR in 4 cases, FISH 
      only in 7 cases, and PCR only in 4 cases. By using multiplex PCR, 6 cases with 
      atypical/negative flow cytometry results were diagnosed as 4 B-cell lymphomas, 1 
      T-cell lymphoma, and 1 reactive lymph node; and 4 cases that had atypical T cells 
      determined by flow cytometry were diagnosed as reactive. One CD10-negative 
      follicular lymphoma and 2 cases with suspicious flow cytometry results were 
      positive for t(14;18)(q32;q21) by FISH. Forty-five cases had follow-up histology 
      with 3 false-negative findings and no false-positive results. CONCLUSIONS: In 
      this study, multiplex PCR studies for immunoglobulin heavy-chain or T-cell 
      receptor gene rearrangements were useful for demonstrating clonality, and FISH 
      studies were able to detect translocations or gene rearrangements that allowed 
      for the subclassification of B-cell non-Hodgkin lymphomas.
CI  - (c) 2010 American Cancer Society.
FAU - Zhang, Songlin
AU  - Zhang S
AD  - Department of Pathology, Louisiana State University Health Science Center, 1501 
      Kings Highway, Shreveport, LA 71130, USA. szhan2@lsuhsc.edu
FAU - Abreo, Fleurette
AU  - Abreo F
FAU - Lowery-Nordberg, Mary
AU  - Lowery-Nordberg M
FAU - Veillon, Diana M
AU  - Veillon DM
FAU - Cotelingam, James D
AU  - Cotelingam JD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
SB  - IM
MH  - *Biopsy, Fine-Needle
MH  - Chromosome Aberrations
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymph Nodes/*pathology
MH  - Lymphoma/diagnosis/genetics
MH  - Lymphoproliferative Disorders/*diagnosis/genetics
MH  - *Molecular Diagnostic Techniques
MH  - *Polymerase Chain Reaction
EDAT- 2010/03/27 06:00
MHDA- 2010/05/26 06:00
CRDT- 2010/03/27 06:00
PHST- 2010/03/27 06:00 [entrez]
PHST- 2010/03/27 06:00 [pubmed]
PHST- 2010/05/26 06:00 [medline]
AID - 10.1002/cncy.20070 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2010 Apr 25;118(2):105-12. doi: 10.1002/cncy.20070.