PMID- 20344943 OWN - NLM STAT- MEDLINE DCOM- 20100429 LR - 20120409 IS - 0001-6209 (Print) IS - 0001-6209 (Linking) VI - 50 IP - 1 DP - 2010 Jan TI - [Effect of tillage and fertilization on bacterial community structure in different depth of black soil]. PG - 67-75 AB - OBJECTIVE: To analyze the impact of tillage and fertilization on bacterial community structure under different depth in black soil. METHODS: We used denaturing gradient gel electrophoresis (DGGE) profile analysis of bacterial community based on the 16S rRNA gene combining soil physico-chemical characteristics. RESULTS: DGGE profiles showed that tillage and fertilization influenced more on bacterial community structrue in the surface soil than the subsurface and deep soil, and the divergence distance between the tillage soil and the control was about 4%. Subsurface and deep soil affected less compared with the control, and the divergence distance was 2%. The vertical similarity of bacterial community structure between subsurface and the deep soil was higher than that of suface soil. CONCLUSION: Tillage and fertilization affected the surface bacterial community (0-30 cm), whereas little on the deeper soil (> 100 cm) bacterial community. FAU - Gao, Chongyang AU - Gao C AD - School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. cy.gao@yahoo.com.cn FAU - Zhao, Yangguo AU - Zhao Y FAU - Wang, Aijie AU - Wang A FAU - Ren, Nanqi AU - Ren N FAU - Bai, Jie AU - Bai J FAU - Tian, Weijun AU - Tian W FAU - Zhang, Jian AU - Zhang J FAU - Li, Haiyan AU - Li H LA - chi PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Wei Sheng Wu Xue Bao JT - Wei sheng wu xue bao = Acta microbiologica Sinica JID - 21610860R RN - 0 (Fertilizers) RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - *Agriculture MH - Bacteria/classification/genetics/*isolation & purification MH - Electrophoresis, Polyacrylamide Gel MH - *Fertilizers MH - Polymerase Chain Reaction MH - RNA, Ribosomal, 16S/genetics MH - *Soil Microbiology EDAT- 2010/03/30 06:00 MHDA- 2010/04/30 06:00 CRDT- 2010/03/30 06:00 PHST- 2010/03/30 06:00 [entrez] PHST- 2010/03/30 06:00 [pubmed] PHST- 2010/04/30 06:00 [medline] PST - ppublish SO - Wei Sheng Wu Xue Bao. 2010 Jan;50(1):67-75.