PMID- 20345437
OWN - NLM
STAT- MEDLINE
DCOM- 20101215
LR  - 20161125
IS  - 1540-8175 (Electronic)
IS  - 0742-2822 (Linking)
VI  - 27
IP  - 6
DP  - 2010 Jul
TI  - Isoproterenol-induced myocardial injury: a systematic comparison of subcutaneous 
      versus intraperitoneal delivery in a rat model.
PG  - 716-21
LID - 10.1111/j.1540-8175.2009.01107.x [doi]
AB  - INTRODUCTION: Isoproterenol (ISO)-induced myocardial injury is widely used as an 
      experimental animal model; however, the optimal route of delivery, i.e., 
      subcutaneous (SC) versus intraperitoneal (IP) has not been clarified. We 
      systematically compared changes in cardiac function (echocardiography, Doppler 
      and strain imaging) and exercise capacity induced by ISO via SC versus IP 
      delivery. METHODS: Twelve rats were used in this study and classified into three 
      groups: Control (n = 2), SC-ISO (n = 5), and IP-ISO (n = 5), each receiving 
      serial injections of ISO (100 mcg/kg) for 5 days (days 1-5). All rats underwent 
      echocardiographic analysis of left ventricular function and functional capacity 
      (FC) assessment on a treadmill protocol at baseline and post treatment. Hearts 
      were excised and weighed at the end of the study. RESULTS: Left ventricular (LV) 
      systolic and diastolic dysfunctions were adequately induced by both SC and IP 
      delivery: > or =13% reduction in fractional shortening, > or =12% increase in 
      wall motion score index, and > or =35% increase in myocardial performance index; 
      > or =49% increase in E/A ratio; > or =9% decline in anterior wall tissue 
      velocity; > or =12% decline in circumferential and radial tissue strain and 
      strain rates; > or =20% decline in FC; and > or =40% increase in 
      echocardiographic LV mass and gross heart weight in both groups. CONCLUSION: 
      Short-duration ISO administration with serial injections via SC and IP routes 
      induces significant myocardial dysfunction and impairs FC with few differences 
      between both modalities.
FAU - George, Jon C
AU  - George JC
AD  - Cardiovascular Research Center, Temple University School of Medicine, 
      Philadelphia, Pennsylvania, USA. jcgeorgemd@hotmail.com
FAU - Liner, Ann
AU  - Liner A
FAU - Hoit, Brian D
AU  - Hoit BD
LA  - eng
PT  - Journal Article
DEP - 20100325
PL  - United States
TA  - Echocardiography
JT  - Echocardiography (Mount Kisco, N.Y.)
JID - 8511187
RN  - 0 (Cardiotonic Agents)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Animals
MH  - Cardiotonic Agents/administration & dosage
MH  - *Disease Models, Animal
MH  - Female
MH  - Heart Injuries/*chemically induced/*diagnostic imaging
MH  - Humans
MH  - Injections, Intraperitoneal
MH  - Injections, Subcutaneous
MH  - Isoproterenol/*administration & dosage
MH  - Rats
MH  - Rats, Nude
MH  - Ultrasonography
EDAT- 2010/03/30 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/03/30 06:00
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - ECHO1107 [pii]
AID - 10.1111/j.1540-8175.2009.01107.x [doi]
PST - ppublish
SO  - Echocardiography. 2010 Jul;27(6):716-21. doi: 10.1111/j.1540-8175.2009.01107.x. 
      Epub 2010 Mar 25.