PMID- 20345531
OWN - NLM
STAT- MEDLINE
DCOM- 20101025
LR  - 20110425
IS  - 1532-950X (Electronic)
IS  - 0161-3499 (Linking)
VI  - 39
IP  - 4
DP  - 2010 Jun
TI  - The effect of uniaxial cyclic tensile load on gene expression in canine cranial 
      cruciate ligamentocytes.
PG  - 433-43
LID - 10.1111/j.1532-950X.2010.00679.x [doi]
AB  - OBJECTIVE: To determine the effects of uniaxial cyclic tensile load amplitude and 
      duration on gene expression in cranial cruciate ligamentocytes cultured in 
      monolayer. STUDY DESIGN: In vitro experimental study. ANIMALS: Adult dogs (n=9) 
      weighing 20-35 kg. METHODS: Cranial cruciate ligaments (CCL, n=18) were 
      aseptically collected, diced, digested using clostridial collagenase, and primary 
      monolayer cultures were established. CCL cells were seeded at a concentration of 
      3 x 10(5) cells/mL onto a specialized collagen membrane. After 24 hours to allow 
      attachment, ligamentocytes were subjected to 0%, 4%, or 8% uniaxial strain for 24 
      or 48 hours using a sinusoidal strain profile at 0.5 Hz. At the end of each time 
      point, the ligamentocytes were harvested and analyzed for collagen 1 (COL1), 
      collagen 3 (COL3), and matrix metalloproteinase-3 (MMP-3) gene expression using 
      reverse transcriptase real-time polymerase chain reaction. RESULTS: Approximately 
      33% of CCL processed for this study yielded viable cell cultures compared with 
      100% of the medial collateral ligaments processed. For CCL cells under uniaxial 
      strain, gene expression for COL1 was variable, but higher strains and longer time 
      in culture resulted in increased COL1 expression. There were no significant 
      differences found for COL3 at any time point or between strain regimens. In 
      general, MMP-3 gene expression was increased early in tissue culture and at 
      higher strains. CONCLUSIONS: COL1 and MMP-3 gene expression can be influenced by 
      amplitude and duration of strain on CCL cells in monolayer culture. CLINICAL 
      RELEVANCE: These data have implications for modeling and understanding canine 
      cruciate ligament pathophysiology. In particular, MMP-3 could serve as a 
      potential preventative or therapeutic target in cruciate disease.
FAU - Breshears, Lee A
AU  - Breshears LA
AD  - Comparative Orthopaedic Laboratory, University of Missouri-Columbia, Columbia, 
      MO, USA. lbreshears@aescparker.com
FAU - Cook, James L
AU  - Cook JL
FAU - Stoker, Aaron M
AU  - Stoker AM
FAU - Fox, Derek B
AU  - Fox DB
FAU - Luther, Jill K
AU  - Luther JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100325
PL  - United States
TA  - Vet Surg
JT  - Veterinary surgery : VS
JID - 8113214
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type III)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament/chemistry/cytology/*metabolism/physiology
MH  - Cells, Cultured
MH  - Collagen Type I/analysis/biosynthesis
MH  - Collagen Type III/analysis/biosynthesis
MH  - Dogs
MH  - Gene Expression Regulation/*physiology
MH  - Matrix Metalloproteinase 3/analysis/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - *Weight-Bearing/physiology
EDAT- 2010/03/30 06:00
MHDA- 2010/10/26 06:00
CRDT- 2010/03/30 06:00
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/10/26 06:00 [medline]
AID - VSU00679 [pii]
AID - 10.1111/j.1532-950X.2010.00679.x [doi]
PST - ppublish
SO  - Vet Surg. 2010 Jun;39(4):433-43. doi: 10.1111/j.1532-950X.2010.00679.x. Epub 2010 
      Mar 25.