PMID- 20345569
OWN - NLM
STAT- MEDLINE
DCOM- 20101022
LR  - 20101004
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 50
IP  - 8
DP  - 2010 Aug
TI  - A comparison of two robotic platforms to screen plateletpheresis donors for HLA 
      antibodies as part of a transfusion-related acute lung injury mitigation 
      strategy.
PG  - 1766-77
LID - 10.1111/j.1537-2995.2010.02626.x [doi]
AB  - BACKGROUND: Efforts to minimize white blood cell alloantibodies, responsible for 
      transfusion-related acute lung injury (TRALI) in components with high-volume 
      single-donor plasma include consideration of plateletpheresis donor screening for 
      human leukocyte antigen (HLA) antibodies. High-throughput screening platforms 
      make this feasible for large blood centers. Which platform to use, donor 
      subgroups to screen, characteristics of detected antibodies, and operational 
      impact of deferring reactive donors are important questions. STUDY DESIGN AND 
      METHODS: We screened 2462 plateletpheresis donor sera for HLA antibodies on 
      automated instruments using HLA Class I and II enzyme-linked immunosorbent assays 
      (ELISA) or a mixed Class I/II Luminex flow analyzer. Screen-reactive samples were 
      further tested by manual Luminex single-antigen assay to determine antibody 
      specificity, estimated corresponding antigen frequency, and signal strength. 
      RESULTS: Alloexposed females had the highest reactivity rate on both platforms 
      (21.0%), with much lower rates for nonexposed individuals or transfused males 
      (1.4%-5.4%). Increasing parity and more recent pregnancy increased their 
      likelihood of screen reactivity. Deferring screen-reactive parous females would 
      result in at least a 4.8% plateletpheresis donor base decrement. Supplemental 
      testing showed higher rates of nonspecific or natural antibodies in ELISA 
      screen-reactive alloexposed females (2.5%) than Luminex (0%). Both assays were 
      more likely to identify antibodies directed against a larger number of HLA 
      antigens and/or of presumed higher titer in alloexposed donors. CONCLUSION: A 
      strategy screening only parous female donors is reasonable. Both automated HLA 
      antibody detection platforms are easy to use and preferentially identify 
      alloexposed individuals with antibodies of presumed higher titer directed against 
      more recipient HLA antigens.
CI  - (c) 2010 American Association of Blood Banks.
FAU - Vassallo, Ralph R
AU  - Vassallo RR
AD  - American Red Cross, Penn-Jersey Region, Philadelphia, Pennsylvania 19123, USA. 
      vassallor@usa.redcross.org
FAU - Hsu, Susan
AU  - Hsu S
FAU - Einarson, Mindy
AU  - Einarson M
FAU - Barone, Jonathan
AU  - Barone J
FAU - Brodsky, Jaye
AU  - Brodsky J
FAU - Moroff, Gary
AU  - Moroff G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20100312
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Donor Selection/*methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Isoantibodies/*blood
MH  - Male
MH  - Middle Aged
MH  - *Plateletpheresis
MH  - *Robotics
EDAT- 2010/03/30 06:00
MHDA- 2010/10/23 06:00
CRDT- 2010/03/30 06:00
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/10/23 06:00 [medline]
AID - TRF2626 [pii]
AID - 10.1111/j.1537-2995.2010.02626.x [doi]
PST - ppublish
SO  - Transfusion. 2010 Aug;50(8):1766-77. doi: 10.1111/j.1537-2995.2010.02626.x. Epub 
      2010 Mar 12.