PMID- 20346245
OWN - NLM
STAT- MEDLINE
DCOM- 20100507
LR  - 20170112
IS  - 0392-856X (Print)
IS  - 0392-856X (Linking)
VI  - 28
IP  - 1
DP  - 2010 Jan-Feb
TI  - Enhanced expression of mRNA for FK506-binding protein 5 in bone marrow CD34 
      positive cells in patients with rheumatoid arthritis.
PG  - 87-90
AB  - OBJECTIVE: Recent studies have disclosed that several genes are up-regulated in 
      bone marrow (BM) mononuclear cells from rheumatoid arthritis (RA) patients. 
      However, it remains unclear whether such abnormalities result from systemic 
      inflammation or from abnormalities at stem cell level. The current study 
      therefore examined the expression of several representative genes, including 
      amphiregulin (AREG), chemokine receptor 4 (CXCR4), and FK506-binding protein 5 
      (FKBP5) in RA BM CD34+ cells. METHODS: BM samples were obtained from 52 patients 
      with RA and 35 patients with osteroarthritis (OA) during joint operations. CD34+ 
      cells were purified from the BM mononuclear cells by positive selection with 
      magnetic beads. The mRNA expression for AREG, CXCR4, and FKBP5 was measured using 
      quantitative real-time PCR. RESULTS: The expression of mRNA for FKBP5, but not 
      that of AREG or CXCR4, was significantly higher in RA BM CD34+ cells than in OA 
      BM CD34+ cells. The FKBP5 mRNA expression level was not correlated with serum CRP 
      or treatment. In addition, tumour necrosis factor-alpha did not enhance the 
      expression of FKBP5 mRNA in BM CD34+ cells from healthy donors. CONCLUSION: The 
      results suggest that the enhanced expression of FKBP5 in BM CD34+ cells might be 
      an intrinsic abnormality of RA BM CD34+ cells, whereas the enhanced expression of 
      AREG and CXCR4 in BM mononuclear cells might be secondary to systemic 
      inflammation.
FAU - Matsushita, R
AU  - Matsushita R
AD  - Department of Rheumatology and Infectious Diseases, Kitasato University School of 
      Medicine, Kanagawa, Japan.
FAU - Hashimoto, A
AU  - Hashimoto A
FAU - Tomita, T
AU  - Tomita T
FAU - Yoshitawa, H
AU  - Yoshitawa H
FAU - Tanaka, S
AU  - Tanaka S
FAU - Endo, H
AU  - Endo H
FAU - Hirohata, S
AU  - Hirohata S
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Clin Exp Rheumatol
JT  - Clinical and experimental rheumatology
JID - 8308521
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Antigens, CD34)
RN  - 0 (CXCR4 protein, human)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 5.2.1.- (Tacrolimus Binding Proteins)
RN  - EC 5.2.1.8 (tacrolimus binding protein 5)
SB  - IM
MH  - Aged
MH  - Amphiregulin
MH  - Antigens, CD34/metabolism
MH  - Arthritis, Rheumatoid/*physiopathology
MH  - Bone Marrow Cells/cytology/drug effects/*physiology
MH  - Cells, Cultured
MH  - EGF Family of Proteins
MH  - Female
MH  - Gene Expression/physiology
MH  - Glycoproteins/genetics
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/genetics
MH  - Male
MH  - Middle Aged
MH  - Osteoarthritis/physiopathology
MH  - RNA, Messenger/metabolism
MH  - Receptors, CXCR4/genetics
MH  - Tacrolimus Binding Proteins/*genetics
MH  - Tumor Necrosis Factor-alpha/metabolism/pharmacology
EDAT- 2010/03/30 06:00
MHDA- 2010/05/08 06:00
CRDT- 2010/03/30 06:00
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/05/08 06:00 [medline]
AID - 283 [pii]
PST - ppublish
SO  - Clin Exp Rheumatol. 2010 Jan-Feb;28(1):87-90.