PMID- 20347132
OWN - NLM
STAT- MEDLINE
DCOM- 20100727
LR  - 20181201
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 19
DP  - 2010 Jul
TI  - Incorporation of tripolyphosphate nanoparticles into fibrous 
      poly(lactide-co-glycolide) scaffolds for tissue engineering.
PG  - 5100-9
LID - 10.1016/j.biomaterials.2010.03.004 [doi]
AB  - Poly(lactide-co-glycolide) (PLGA) has been widely used for scaffolding materials 
      in tissue engineering. It degrades mainly via hydrolysis of the ester bonds into 
      lactic acid and glycolic acid leading to the decrease in pH of the surrounding 
      microenvironment. The current study was designed to quickly neutralize the acidic 
      degradation products of PLGA fibrous scaffolds by incorporating tripolyphosphate 
      (TPP) nanoparticles into PLGA fibers. A homogeneous mixture of PLGA and TPP was 
      first obtained by water-in-oil emulsion-dispersion followed by freeze-drying. The 
      dried blend was melt-spun to yield fibers which were processed into scaffolds and 
      subsequently immersed into phosphate-buffered saline (PBS) to verify the 
      degradation properties. The pH of the saline was monitored for a duration of 80 
      days. The amount of TPP was optimized to obtain a PLGA based scaffolds without 
      acidic degradation problems. Cellular compatibility of the modified and pristine 
      scaffolds was evaluated using rabbit adipose-derived stem cells (rASCs). It was 
      shown that TPP particles within the fibers were roughly 100nm in diameter and 
      mainly located inside fibers instead of on the superficial layer. The acidic 
      degradation of PT-16 and PT-64 (PT-X is termed when the monomer molar ratio of 
      TPP to PLGA was 1:X) was significantly improved as the pH values of their 
      respective solutions were maintained in a well neutralized state during the 
      degradation. PT-64 and PT-16 scaffolds could well support the attachment and 
      proliferation of rASCs. Hence, the incorporation of TPP nanoparticles via an 
      emulsion-dispersion method could be an effective strategy to improve/adjust the 
      acidic degradation of PLGA and further pave the way for clinical applications of 
      such polyesters.
CI  - Copyright 2010 Elsevier Ltd. All rights reserved.
FAU - Xie, Shujun
AU  - Xie S
AD  - National Tissue Engineering Center of China, No.100, Qin Zhou Road, Shanghai 
      200235, China.
FAU - Zhu, Qin
AU  - Zhu Q
FAU - Wang, Bin
AU  - Wang B
FAU - Gu, Huijie
AU  - Gu H
FAU - Liu, Wei
AU  - Liu W
FAU - Cui, Lei
AU  - Cui L
FAU - Cen, Lian
AU  - Cen L
FAU - Cao, Yilin
AU  - Cao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100327
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polyphosphates)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - NU43IAG5BC (triphosphoric acid)
SB  - IM
MH  - Adipocytes/*cytology/*physiology
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Lactic Acid/*chemistry
MH  - Materials Testing
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Particle Size
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polyphosphates/*chemistry
MH  - Rabbits
MH  - Tissue Engineering/*instrumentation/methods
MH  - *Tissue Scaffolds
EDAT- 2010/03/30 06:00
MHDA- 2010/07/28 06:00
CRDT- 2010/03/30 06:00
PHST- 2010/01/04 00:00 [received]
PHST- 2010/03/02 00:00 [accepted]
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/07/28 06:00 [medline]
AID - S0142-9612(10)00344-3 [pii]
AID - 10.1016/j.biomaterials.2010.03.004 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Jul;31(19):5100-9. doi: 10.1016/j.biomaterials.2010.03.004. 
      Epub 2010 Mar 27.