PMID- 20347716
OWN - NLM
STAT- MEDLINE
DCOM- 20110110
LR  - 20211020
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1797
IP  - 6-7
DP  - 2010 Jun-Jul
TI  - Regulation of mitochondrial fission by intracellular Ca2+ in rat ventricular 
      myocytes.
PG  - 913-21
LID - 10.1016/j.bbabio.2010.03.018 [doi]
AB  - Mitochondria are dynamic organelles that constantly undergo fission, fusion, and 
      movement. Increasing evidence indicates that these dynamic changes are 
      intricately related to mitochondrial function, suggesting that mitochondrial form 
      and function are linked. Calcium (Ca2+) is one signal that has been shown to both 
      regulate mitochondrial fission in various cell types and stimulate mitochondrial 
      enzymes involved in ATP generation. However, although Ca2+ plays an important 
      role in adult cardiac muscle cells for excitation-metabolism coupling, little is 
      known about whether Ca2+ can regulate their mitochondrial morphology. Therefore, 
      we tested the role of Ca2+ in regulating cardiac mitochondrial fission. We found 
      that neonatal and adult cardiomyocyte mitochondria undergo rapid and transient 
      fragmentation upon a thapsigargin (TG)- or KCl-induced cytosolic Ca2+ increase. 
      The mitochondrial fission protein, DLP1, participates in this mitochondrial 
      fragmentation, suggesting that cardiac mitochondrial fission machinery may be 
      regulated by intracellular Ca2+ signaling. Moreover, the TG-induced fragmentation 
      was also associated with an increase in reactive oxygen species (ROS) formation, 
      suggesting that activation of mitochondrial fission machinery is an early event 
      for Ca2+-mediated ROS generation in cardiac myocytes. These results suggest that 
      Ca2+, an important regulator of muscle contraction and energy generation, also 
      dynamically regulates mitochondrial morphology and ROS generation in cardiac 
      myocytes.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Hom, Jennifer
AU  - Hom J
AD  - Department of Pediatrics, University of Rochester School of Medicine and 
      Dentistry, Rochester, NY 14642, USA.
FAU - Yu, Tianzheng
AU  - Yu T
FAU - Yoon, Yisang
AU  - Yoon Y
FAU - Porter, George
AU  - Porter G
FAU - Sheu, Shey-Shing
AU  - Sheu SS
LA  - eng
GR  - DK 61991/DK/NIDDK NIH HHS/United States
GR  - R01 HL033333-21/HL/NHLBI NIH HHS/United States
GR  - HL 33333/HL/NHLBI NIH HHS/United States
GR  - R01 HL033333/HL/NHLBI NIH HHS/United States
GR  - R01 DK061991/DK/NIDDK NIH HHS/United States
GR  - R01 HL093671-01A1/HL/NHLBI NIH HHS/United States
GR  - R01 HL093671/HL/NHLBI NIH HHS/United States
GR  - R01 DK061991-06A2/DK/NIDDK NIH HHS/United States
GR  - DK 73858/DK/NIDDK NIH HHS/United States
GR  - R21 DK073858-02/DK/NIDDK NIH HHS/United States
GR  - R01 DK078618/DK/NIDDK NIH HHS/United States
GR  - R21 DK073858/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100327
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 11062-77-4 (Superoxides)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.5.5 (Dnm1l protein, rat)
RN  - EC 3.6.5.5 (Dynamins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Calcium/*metabolism
MH  - Calcium Signaling/drug effects
MH  - Cells, Cultured
MH  - Dynamins
MH  - GTP Phosphohydrolases/metabolism
MH  - In Vitro Techniques
MH  - Microscopy, Electron, Transmission
MH  - Microtubule-Associated Proteins/metabolism
MH  - Mitochondria, Heart/drug effects/*metabolism/ultrastructure
MH  - Mitochondrial Proteins/metabolism
MH  - Myocytes, Cardiac/drug effects/*metabolism/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxides/metabolism
MH  - Thapsigargin/pharmacology
PMC - PMC2891135
MID - NIHMS199418
EDAT- 2010/03/30 06:00
MHDA- 2011/01/11 06:00
PMCR- 2011/06/01
CRDT- 2010/03/30 06:00
PHST- 2009/12/01 00:00 [received]
PHST- 2010/03/19 00:00 [revised]
PHST- 2010/03/19 00:00 [accepted]
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2011/01/11 06:00 [medline]
PHST- 2011/06/01 00:00 [pmc-release]
AID - S0005-2728(10)00124-6 [pii]
AID - 10.1016/j.bbabio.2010.03.018 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):913-21. doi: 
      10.1016/j.bbabio.2010.03.018. Epub 2010 Mar 27.