PMID- 20347932
OWN - NLM
STAT- MEDLINE
DCOM- 20100630
LR  - 20220310
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 475
IP  - 2
DP  - 2010 May 14
TI  - In vitro neural differentiation of bone marrow stromal cells induced by 
      cocultured olfactory ensheathing cells.
PG  - 99-103
LID - 10.1016/j.neulet.2010.03.056 [doi]
AB  - Bone marrow stromal cells (BMSCs) could be induced to differentiate into neural 
      cells under certain conditions, nevertheless, optimal protocols that could be 
      reproducible and reliable in generating transplantable BMSCs in vitro are still 
      not available. We studied for the first time the neural differentiation of BMSCs 
      induced by coculturing with olfactory ensheathing cells (OECs). BMSCs and OECs 
      were isolated from bone marrow and nasal olfactory lamina propria of adult SD 
      rats respectively, then brought to coculture with transwell culture dishes. At 
      various time points (0h, 6h, 12h, 24h, 72h, 1 week and 2 weeks post-coculture), 
      BMSCs were morphologically observed and processed for immunofluorescence and 
      reverse transcription-polymerase chain reaction (RT-PCR). The number of cells 
      assuming neural morphology dramatically increased at 1- and 
      2-week-post-coculture, so as the number of immunoreactive cells labeled by neural 
      markers NSE, beta-III-tubulin, MAP2, GFAP and p75(NTR). Our findings demonstrate 
      that BMSCs can efficiently differentiate into neural cells when coculturing with 
      OECs, and the present protocol provides an alternative neurogenesis pathway for 
      generating sufficient numbers of neural cells from BMSCs.
CI  - Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Ni, Wen-Fei
AU  - Ni WF
AD  - Department of Spine Surgery, The Second Affiliated Hospital of Wenzhou Medical 
      College, China.
FAU - Yin, Li-Hui
AU  - Yin LH
FAU - Lu, Jike
AU  - Lu J
FAU - Xu, Hua-Zi
AU  - Xu HZ
FAU - Chi, Yong-Long
AU  - Chi YL
FAU - Wu, Jian-Bo
AU  - Wu JB
FAU - Zhang, Nu
AU  - Zhang N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100327
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Cell Differentiation
MH  - Coculture Techniques
MH  - Extracellular Matrix Proteins/metabolism
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Nerve Tissue/*cytology
MH  - Olfactory Mucosa/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stromal Cells/cytology
EDAT- 2010/03/30 06:00
MHDA- 2010/07/01 06:00
CRDT- 2010/03/30 06:00
PHST- 2009/12/24 00:00 [received]
PHST- 2010/03/10 00:00 [revised]
PHST- 2010/03/22 00:00 [accepted]
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/07/01 06:00 [medline]
AID - S0304-3940(10)00365-4 [pii]
AID - 10.1016/j.neulet.2010.03.056 [doi]
PST - ppublish
SO  - Neurosci Lett. 2010 May 14;475(2):99-103. doi: 10.1016/j.neulet.2010.03.056. Epub 
      2010 Mar 27.