PMID- 20348039
OWN - NLM
STAT- MEDLINE
DCOM- 20100913
LR  - 20160518
IS  - 1876-7737 (Electronic)
IS  - 1874-3919 (Linking)
VI  - 73
IP  - 8
DP  - 2010 Jun 16
TI  - Mass spectrometric characterization of isoform variants of peanut (Arachis 
      hypogaea) stem lectin (SL-I).
PG  - 1573-86
LID - 10.1016/j.jprot.2010.03.006 [doi]
AB  - Matrix assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass 
      spectrometric (MS) analysis of purified Arachis hypogaea stem lectin (SL-I) and 
      its tryptic digests suggested it to be an isoformic glucose/mannose binding 
      lectin. Two-dimensional gel electrophoresis of SL-I indicated six isoforms 
      (A1-A6), which were confirmed by Western blotting and MALDI-TOF MS analysis. 
      Comparative analysis of peptide mass spectra of the isoforms matched with A. 
      hypogaea lectins with three different accession numbers (Q43376_ARAHY, 
      Q43377_ARAHY, Q70DJ5_ARAHY). Tandem mass spectrometric (MS/MS) analysis of 
      tryptic peptides revealed these to be isoformic variants with altered amino acid 
      sequences. Among the peptides, the peptide T12 showed major variation. The 
      (199)Val-Ser-Tyr-Asn(202) sequence in peptide T12 of A1 and A2 was replaced by 
      (199)Leu-Ser-His-Glu(202) in A3 and A4 (T12') while in A5 and A6 this sequence 
      was (199)Val-Ser-Tyr-Val(202) (T12''). Peptide T1 showed the presence of (10)Asn 
      in the isoforms A1-A5 while in A6 this amino acid was replaced by (10)Lys (T1'). 
      Overall amino acid sequence as identified by MS/MS showed a high degree of 
      similarity between A1, A2 and among A3, A4, A5. Carbohydrate binding domain and 
      adenine binding site seem to be conserved.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Agrawal, Praveen
AU  - Agrawal P
AD  - Proteomics and Structural Biology Division, Institute of Genomics and Integrative 
      Biology, Delhi, India.
FAU - Kumar, Saravanan
AU  - Kumar S
FAU - Das, Hasi Rani
AU  - Das HR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100327
PL  - Netherlands
TA  - J Proteomics
JT  - Journal of proteomics
JID - 101475056
RN  - 0 (Plant Lectins)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arachis/*chemistry
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Plant Lectins/*chemistry
MH  - Plant Stems/chemistry
MH  - Protein Isoforms/analysis
MH  - Sequence Alignment
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
EDAT- 2010/03/30 06:00
MHDA- 2010/09/14 06:00
CRDT- 2010/03/30 06:00
PHST- 2009/10/28 00:00 [received]
PHST- 2010/02/11 00:00 [revised]
PHST- 2010/03/10 00:00 [accepted]
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/09/14 06:00 [medline]
AID - S1874-3919(10)00080-1 [pii]
AID - 10.1016/j.jprot.2010.03.006 [doi]
PST - ppublish
SO  - J Proteomics. 2010 Jun 16;73(8):1573-86. doi: 10.1016/j.jprot.2010.03.006. Epub 
      2010 Mar 27.