PMID- 20348138
OWN - NLM
STAT- MEDLINE
DCOM- 20101215
LR  - 20171116
IS  - 1755-3245 (Electronic)
IS  - 0008-6363 (Linking)
VI  - 87
IP  - 4
DP  - 2010 Sep 1
TI  - Gender-specific effects of caloric restriction on the balance of vascular nitric 
      oxide and superoxide radical.
PG  - 751-9
LID - 10.1093/cvr/cvq095 [doi]
AB  - AIMS: Caloric restriction (CR) and female gender attenuate oxidative damage and 
      improve vascular endothelium-dependent relaxation (EDR). Multiple mechanisms that 
      ameliorate vascular O(2)(*-) could enhance the NO(*)/O(2)(*-) balance and thus 
      improve EDR. The aim of this study is to compare the effects of short-term (2 
      weeks) CR and gender on molecular mechanisms involved in NO(*)/O(2)(*-) balance 
      and EDR. METHODS AND RESULTS: Wistar rats (8 weeks old) of both genders were fed 
      ad libitum (control) or were subjected to CR (60% of food intake of controls) for 
      2 weeks. Plasma levels of NO(*), insulin, and ghrelin, EDR, vascular NO(*) and 
      O(2)(*-) production, as well as endothelial NO(*) synthase (eNOS) and NADPH 
      oxidase (Nox) expression were examined and analysed. CR improved EDR and vascular 
      NO(*) levels and ameliorated NADPH-sensitive O(2)(*-) production in male rats 
      more than in females. Both CR and female gender reduced mRNA expression of Nox1 
      and Nox p22phox (p22phox); however, CR reduced Nox4 and p47phox only in males. 
      Protein expression studies showed that CR enhanced eNOS and reduced Nox4 only in 
      males. CONCLUSION: Short-term CR improved the NO(*)/O(2)(*-) balance by lowering 
      vascular O(2)(*-) production through decreased expression of Nox in males, thus 
      enhancing bioactive NO(*) levels and EDR. In this regard, CR shifted the state of 
      vascular NO(*)/O(2)(*-) balance in males to a state similar to that in females.
FAU - Chou, Shih-Hsuan
AU  - Chou SH
AD  - Department of Physiology and Pharmacology, Chang Gung University, Taoyuan, 
      Taiwan, Republic of China.
FAU - Lee, Yung-Chen
AU  - Lee YC
FAU - Huang, Chiu-Feng
AU  - Huang CF
FAU - Wang, Yu-Ren
AU  - Wang YR
FAU - Yu, Huang-Ping
AU  - Yu HP
FAU - Lau, Ying-Tung
AU  - Lau YT
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100325
PL  - England
TA  - Cardiovasc Res
JT  - Cardiovascular research
JID - 0077427
RN  - 0 (Ghrelin)
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vasodilator Agents)
RN  - 11062-77-4 (Superoxides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - Animals
MH  - Aorta/drug effects/*metabolism
MH  - *Caloric Restriction
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gene Expression Regulation, Enzymologic
MH  - Ghrelin/blood
MH  - Insulin/blood
MH  - Male
MH  - NADPH Oxidases/genetics/metabolism
MH  - Nitric Oxide/blood/*metabolism
MH  - Nitric Oxide Synthase Type III/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Sex Factors
MH  - Superoxides/*metabolism
MH  - Time Factors
MH  - *Vasodilation
MH  - Vasodilator Agents/pharmacology
EDAT- 2010/03/30 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/03/30 06:00
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - cvq095 [pii]
AID - 10.1093/cvr/cvq095 [doi]
PST - ppublish
SO  - Cardiovasc Res. 2010 Sep 1;87(4):751-9. doi: 10.1093/cvr/cvq095. Epub 2010 Mar 
      25.