PMID- 2034887
OWN - NLM
STAT- MEDLINE
DCOM- 19910624
LR  - 20190912
IS  - 0300-9130 (Print)
IS  - 0300-9130 (Linking)
VI  - 191
IP  - 1
DP  - 1991
TI  - Determination of 2-fluoro-2-deoxy-D-glucose uptake and ATP level for evaluating 
      drug effects in neoplastic cells.
PG  - 27-35
AB  - The glucose analogue 2-fluoro-2-deoxy-D-glucose (FDG) was used to study 
      chemosensitivity of two human ovarian cancer cell lines and of murine L1210 
      cells. Cell viability was determined by measuring intracellular adenosine 
      triphosphate (ATP) with a bioluminescence method, which has been shown to 
      correlate closely with trypan blue, stem cell, and [3H]TdR assays. All three cell 
      lines were sensitive to cytostatic drugs, which exerted a parallel decrease in 
      the intracellular FDG and ATP levels. The two measures correlated positively (r = 
      0.66, P less than 0.001), indicating that FDG uptake is closely linked with ATP 
      production. Relatively low hexokinase (HK)-to-glucose 6-phosphatase (HK/G6-Pase) 
      ratios were measured, which suggests that the metabolic trapping of FDG 
      6-phosphate within the cytosol is incomplete. Apparently, these cell lines may 
      not depend exclusively on glycolysis for their energy requirement. We conclude 
      that cell killing caused by cytostatic drugs is associated with a decreased ATP 
      content and FDG uptake. This indicates that not only ATP but also FDG may be used 
      to study drug effects in vitro.
FAU - Minn, H
AU  - Minn H
AD  - Department of Oncology and Radiotherapy, University Central Hospital of Turku, 
      Finland.
FAU - Kangas, L
AU  - Kangas L
FAU - Knuutila, V
AU  - Knuutila V
FAU - Paul, R
AU  - Paul R
FAU - Sipila, H
AU  - Sipila H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Res Exp Med (Berl)
JT  - Research in experimental medicine. Zeitschrift fur die gesamte experimentelle 
      Medizin einschliesslich experimenteller Chirurgie
JID - 0324736
RN  - 0 (Antineoplastic Agents)
RN  - 0Z5B2CJX4D (Fluorodeoxyglucose F18)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9G2MP84A8W (Deoxyglucose)
SB  - IM
MH  - Adenosine Triphosphate/*analysis
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Cell Survival/drug effects
MH  - Deoxyglucose/*analogs & derivatives/metabolism
MH  - *Drug Screening Assays, Antitumor
MH  - Energy Metabolism
MH  - Female
MH  - Fluorodeoxyglucose F18
MH  - Glycolysis/drug effects
MH  - Humans
MH  - Leukemia L1210/pathology
MH  - Luminescent Measurements
MH  - Mice
MH  - Ovarian Neoplasms/pathology
MH  - Tumor Cells, Cultured/*metabolism
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1007/BF02576657 [doi]
PST - ppublish
SO  - Res Exp Med (Berl). 1991;191(1):27-35. doi: 10.1007/BF02576657.