PMID- 20349081
OWN - NLM
STAT- MEDLINE
DCOM- 20101007
LR  - 20211020
IS  - 1439-6327 (Electronic)
IS  - 1439-6319 (Linking)
VI  - 109
IP  - 5
DP  - 2010 Jul
TI  - Matrix metalloprotease-3 and tissue inhibitor of metalloprotease-1 mRNA and 
      protein levels are altered in response to traumatic skeletal muscle injury.
PG  - 963-72
LID - 10.1007/s00421-010-1435-5 [doi]
AB  - The purpose of this study was to characterize the time course of matrix 
      metalloprotease-3 (MMP-3) and tissue inhibitor of metalloprotease-1 (TIMP-1) 
      expression in mouse tibialis anterior (TA) muscle post-injury. Mice were 
      anesthetized, the TA muscle exposed, and injury induced by applying a cold steel 
      probe (-79 degrees C) to the muscle for 10 s. Muscle was collected from uninjured 
      and injured legs at 3, 10, 24, 48, and 72 h post-injury. qRT-PCR, immunoblotting, 
      and immunohistochemistry were used to quantify/localize MMP-3 and TIMP-1. MMP-3 
      transcripts increased 19- and 12-fold, 10 and 24 h post-injury (p < 0.01), 
      respectively. TIMP-1 transcript levels increased 9-, 34-, and 60-fold, 10, 24, 
      and 48 h post-injury (p = 0.01), respectively, with a subsequent decrease 72 h 
      post-injury (p < 0.01). Protein levels of the pro-form of MMP-3 increased within 
      3 h post-injury and remained elevated (p < 0.05). Active MMP-3 decreased over 
      time, reaching a 72% decrease 72 h post-injury (p < 0.05). TIMP-1 protein 
      decreased 75% within 3 h post-injury, returning to baseline by 72 h post-injury. 
      In response to injury, injured skeletal muscle preferentially produces increased 
      levels of the latent form of the MMP-3 protein with a concomitant decrease in the 
      active form, and a significant decrease in TIMP-1 expression. The altered pattern 
      of MMP-3/TIMP-1 expression may be due to alterations in post-transcriptional 
      mechanisms that are responsible for specific regulation of the MMP-3/TIMP-1 
      system. These data suggest that there is a disproportionate regulation of the 
      MMP-3/TIMP-1 system following traumatic injury and this response may contribute 
      to impaired extracellular matrix remodeling.
FAU - Urso, Maria L
AU  - Urso ML
AD  - U.S. Army Research Institute of Environmental Medicine, 42 Kansas St, Building 
      42, Natick, MA 01760, USA. maria.urso@us.army.mil
FAU - Szelenyi, Eric R
AU  - Szelenyi ER
FAU - Warren, Gordon L
AU  - Warren GL
FAU - Barnes, Brian R
AU  - Barnes BR
LA  - eng
PT  - Journal Article
DEP - 20100327
PL  - Germany
TA  - Eur J Appl Physiol
JT  - European journal of applied physiology
JID - 100954790
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Male
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - Muscle, Skeletal/*injuries/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - Regeneration
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinase-1/*metabolism
MH  - *Wounds and Injuries
EDAT- 2010/03/30 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/03/30 06:00
PHST- 2010/03/05 00:00 [accepted]
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
AID - 10.1007/s00421-010-1435-5 [doi]
PST - ppublish
SO  - Eur J Appl Physiol. 2010 Jul;109(5):963-72. doi: 10.1007/s00421-010-1435-5. Epub 
      2010 Mar 27.