PMID- 20350359
OWN - NLM
STAT- MEDLINE
DCOM- 20100719
LR  - 20161125
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 19
IP  - 2
DP  - 2010
TI  - Purification of mouse bone marrow-derived stem cells promotes ex vivo neuronal 
      differentiation.
PG  - 193-202
LID - 10.3727/096368910X492599 [doi]
AB  - The main objective of this study is to test the potential of specific populations 
      of mouse bone marrow-derived stem cells (BMSCs) to differentiate into the 
      neuronal cell lineage. Bone marrow of 33 mice was aspirated under general 
      anesthesia. The collected marrows were analyzed for cell counts, compositions, 
      and percentages of different stem cell types. We used the Midi MACS magnetic 
      separator to purify specific populations of stem cells from the aspirated bone 
      marrow. Cells were analyzed using flow cytometry. We assessed the presence of 
      stem cell antigen-1 (Sca-1(+)) and prominin-1(+) cells in the cellular fraction 
      that was depleted of lineage-committed cells (lineage(-)). Both purified and 
      nonpurified cells were cultured ex vivo using specific growth media with factors 
      that drive the cells to differentiate into the neuroglial cell types. Cells were 
      then analyzed by flow cytometry for expression of specific neuronal markers. Our 
      results showed that there was an increase of Sca-1(+) and prominin-1(+) cells in 
      the lineage(-) fraction over the unpurified BM. After lineage depletion, the 
      percentages of Sca-1(+) and prominin-1(+) cells increased from 4.9% and 2.6%, up 
      to 76.1% and 59%, respectively. Unpurified mouse BM differentiated into 
      fibroblasts, whereas Sca-1(+) cells were able to generate astrocytes. 
      Interestingly, purified prominin-1(+) cells were able to generate neuronal cells. 
      Purification of adult bone marrow-derived stem cells enhances their potentiality 
      for differentiating into specific neuronal cell lineages.
FAU - Jamous, Mohammad
AU  - Jamous M
AD  - Department of Neurosciences, Jordan University of Science and Technology, Irbid, 
      Jordan. mojamous@yahoo.com <mojamous@yahoo.com>
FAU - Al-Zoubi, Adeeb
AU  - Al-Zoubi A
FAU - Khabaz, Mohamad Nidal
AU  - Khabaz MN
FAU - Khaledi, Rana
AU  - Khaledi R
FAU - Al Khateeb, Mohammad
AU  - Al Khateeb M
FAU - Al-Zoubi, Zaid
AU  - Al-Zoubi Z
LA  - eng
PT  - Journal Article
DEP - 20100326
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (AC133 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Ly)
RN  - 0 (Biomarkers)
RN  - 0 (Glycoproteins)
RN  - 0 (Ly6a protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptides)
RN  - 0 (Prom1 protein, mouse)
SB  - IM
MH  - AC133 Antigen
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Ly/metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/cytology/*physiology
MH  - Cell Lineage
MH  - Cell Separation/*methods
MH  - Cells, Cultured
MH  - Glycoproteins/metabolism
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Neurogenesis
MH  - Neurons/cytology/*physiology
MH  - Peptides/metabolism
MH  - Stem Cells/cytology/*physiology
EDAT- 2010/03/31 06:00
MHDA- 2010/07/20 06:00
CRDT- 2010/03/31 06:00
PHST- 2010/03/31 06:00 [entrez]
PHST- 2010/03/31 06:00 [pubmed]
PHST- 2010/07/20 06:00 [medline]
AID - ct2333jamous [pii]
AID - 10.3727/096368910X492599 [doi]
PST - ppublish
SO  - Cell Transplant. 2010;19(2):193-202. doi: 10.3727/096368910X492599. Epub 2010 Mar 
      26.