PMID- 20351456
OWN - NLM
STAT- MEDLINE
DCOM- 20100616
LR  - 20191210
IS  - 1972-2680 (Electronic)
IS  - 1972-2680 (Linking)
VI  - 4
IP  - 3
DP  - 2010 Mar 29
TI  - Correlation of Trichomonas vaginalis to bacterial vaginosis: a laboratory-based 
      study.
PG  - 156-63
AB  - BACKGROUND: This study aimed to define the occurrence of different organisms 
      causing vulvovaginitis; to evaluate different laboratory methods used for 
      diagnosis of Trichomonas vaginalis (T. vaginalis); and to evaluate the direct 
      score system and clue cell method compared with culture for diagnosis of 
      bacterial and T. vaginalis vaginosis. METHODOLOGY: Clinical and laboratory 
      evaluations were performed for 110 patients. Laboratory methods used for 
      bacteriological diagnosis were direct Gram staining for clue cells and scoring by 
      Nugent score system and bacterial culture. T. vaginalis was identified by wet 
      mount microscopic examination, culture, direct Gram, Giemsa staining and acridine 
      orange (AO). RESULTS: The Nugent score method revealed that the sensitivity and 
      specificity for diagnosis of vaginal discharge by direct rapid microscopic 
      methods were 30% and 80% and for clue cells sensitivity and specificity were 37% 
      and 75% respectively for diagnosis of bacterial vaginosis compared to culture. 
      For diagnosis of T. vaginalis, the Nugent score method revealed that the 
      sensitivity and specificity were 60% and 90% respectively, and for clue cells 75% 
      and 80% respectively. For microcopic methods used for T. vaginalis only, the Gram 
      stain and Giemsa stain sensitivities were poor (15.2% and 48.5%, respectively). 
      Wet mount showed reasonable sensitivity of 75.8%. Acridine orange sensitivity was 
      93.9% and specificity was 97.5%, CONCLUSION: Prevalent pathogens associated with 
      vaginitis were (Gardnerella vaginalis) G. vaginalis, T. vaginalis and Mycoplasma 
      hominis (M. hominis). Wet mount microscopic examination, acridine orange, and 
      high Nugent score were found as rapid and sensitive methods for diagnosis of T. 
      vaginalis.
FAU - El Sayed Zaki, Maysaa
AU  - El Sayed Zaki M
AD  - Departments of Clinical Pathology, Faculty of Medicine, Mansoura University, 
      Mansoura, Egypt. may_s65@hotmail.com
FAU - Raafat, Douaa
AU  - Raafat D
FAU - El Emshaty, Wafaa
AU  - El Emshaty W
FAU - Azab, Manar Sobh
AU  - Azab MS
FAU - Goda, Hossam
AU  - Goda H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20100329
PL  - Italy
TA  - J Infect Dev Ctries
JT  - Journal of infection in developing countries
JID - 101305410
RN  - 0 (Azure Stains)
RN  - 0 (Gram's stain)
RN  - 0 (Phenazines)
RN  - F30N4O6XVV (Acridine Orange)
RN  - J4Z741D6O5 (Gentian Violet)
SB  - IM
MH  - Acridine Orange
MH  - Adult
MH  - Azure Stains
MH  - Female
MH  - Gardnerella vaginalis/growth & development/isolation & purification
MH  - Gentian Violet
MH  - Humans
MH  - Middle Aged
MH  - Mycoplasma hominis/growth & development/isolation & purification
MH  - Phenazines
MH  - Sensitivity and Specificity
MH  - Staining and Labeling/methods
MH  - Trichomonas vaginalis/growth & development/*isolation & purification
MH  - Vaginal Discharge/etiology
MH  - Vaginal Smears
MH  - Vaginosis, Bacterial/complications/*diagnosis/*microbiology
EDAT- 2010/03/31 06:00
MHDA- 2010/06/17 06:00
CRDT- 2010/03/31 06:00
PHST- 2009/08/15 00:00 [received]
PHST- 2010/01/08 00:00 [accepted]
PHST- 2009/12/30 00:00 [revised]
PHST- 2010/03/31 06:00 [entrez]
PHST- 2010/03/31 06:00 [pubmed]
PHST- 2010/06/17 06:00 [medline]
AID - 10.3855/jidc.434 [doi]
PST - epublish
SO  - J Infect Dev Ctries. 2010 Mar 29;4(3):156-63. doi: 10.3855/jidc.434.