PMID- 20352932
OWN - NLM
STAT- MEDLINE
DCOM- 20131212
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 6
DP  - 2009 Nov
TI  - [Determination of 61 central nervous system drugs in plasma by protein 
      precipitation-high performance liquid chromatography].
PG  - 787-93
AB  - A method was established for the determination of 61 central nervous system drugs 
      in plasma by using protein precipitation combined with high performance liquid 
      chromatography-diode array detection (HPLC-DAD). A volume of 1.5 mL acetonitrile 
      was added into 1 mL plasma, after vortex, centrifugation and filtration, the 
      supernatant was directly injected into HPLC. The separation was performed on an 
      Agilent TC-C18 column (250 mm x 4.6 mm, 5 microm) with acetonitrile and phosphate 
      buffer solution as mobile phase by gradient elution at a flow rate of 1.5 mL/min. 
      The detection wavelength was 210 nm; full spectra were recorded from 200-364 nm. 
      The recoveries of 61 drugs were larger than 80% with the relative standard 
      deviations (RSDs) ranged from 0.94% to 11.23%. The protein precipitation method 
      is simple, rapid, low-cost with good recoveries, reproducibility and suitable for 
      the general pretreatment of the systematic toxicological analysis (STA) of the 61 
      drugs.
FAU - Zhang, Yin
AU  - Zhang Y
AD  - Department of Pharmacy, the Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou 362000, China.
FAU - Chen, Chonghong
AU  - Chen C
FAU - Lin, Ling
AU  - Lin L
FAU - Chen, Yinong
AU  - Chen Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Central Nervous System Agents)
SB  - IM
MH  - Central Nervous System Agents/*blood/isolation & purification/pharmacokinetics
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
EDAT- 2010/04/01 06:00
MHDA- 2013/12/18 06:00
CRDT- 2010/04/01 06:00
PHST- 2010/04/01 06:00 [entrez]
PHST- 2010/04/01 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 Nov;27(6):787-93.