PMID- 20352935
OWN - NLM
STAT- MEDLINE
DCOM- 20131212
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 6
DP  - 2009 Nov
TI  - [Determination of six p-hydroxybenzoates in fruits and jams using solid-phase 
      extraction-high performance liquid chromatography].
PG  - 804-8
AB  - A method was developed for the determination of 6 p-hydroxybenzoates (methyl 
      p-hydroxybenzoate (MHB), ethyl p-hydroxybenzoate (EHB), isopropyl 
      p-hydroxybenzoate (IPHB), propyl p-hydroxybenzoate (PHB), isobutyl 
      p-hydroxybenzoate (IBHB) and butyl p-hydroxybenzoate (BHB)) in fruits and jams 
      using the combination of solid-phase extraction and high performance liquid 
      chromatography (SPE-HPLC). Two different extraction solutions and three different 
      mobile phases were tested for p-hydroxybenzoates analysis, and finally ethanol 
      was used as the extraction solvent and methanol-citric acid buffer was selected 
      as the mobile phase. The sample was extracted, and purified by an Oasis HLB 
      solid-phase extraction cartridge, then separated on a Symmetry-C18 column and 
      detected at the wave length of 258 nm. The results showed that all the 
      calibration graphs were linear in the concentration range of 0.1 -20.0 mg/L (r = 
      0.9999). The detection limits and quantification limits were 0.1 mg/kg (S/N = 3) 
      and 0.3 mg/kg (S/N = 10) respectively for MHB, EHB, IPHB and PHB, 0.2 mg/kg (S/N 
      = 3) and 0.6 mg/kg (S/N = 10) respectively for IBHB and BHB. The average 
      recoveries were between 82.8% and 115.5% with the relative standard deviations 
      (RSDs) of 0.2%-6.8% (n = 6). The method is simple, rapid, sensitive and 
      reproducible, and can be used for the routine analysis of the p-hydroxybenzoates 
      in fruits and jams.
FAU - Chen, Ai
AU  - Chen A
AD  - National Marine Food Quality Supervision and Testing Center, Zhoushan 316021, 
      China.
FAU - He, Qiaosang
AU  - He Q
FAU - Wang, Pingya
AU  - Wang P
FAU - Zhou, Yong
AU  - Zhou Y
FAU - Huang, Li
AU  - Huang L
FAU - Xu, Zhenjian
AU  - Xu Z
FAU - Zhao, Hua
AU  - Zhao H
FAU - Zhang, Weiying
AU  - Zhang W
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Hydroxybenzoates)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fruit/*chemistry
MH  - Hydroxybenzoates/*analysis
MH  - Solid Phase Extraction/*methods
EDAT- 2010/04/01 06:00
MHDA- 2013/12/18 06:00
CRDT- 2010/04/01 06:00
PHST- 2010/04/01 06:00 [entrez]
PHST- 2010/04/01 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 Nov;27(6):804-8.