PMID- 20352937
OWN - NLM
STAT- MEDLINE
DCOM- 20131212
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 6
DP  - 2009 Nov
TI  - [Determination of 5 polyether antibiotics in chicken tissues by liquid 
      chromatography-electrospray ionization tandem mass spectrometry].
PG  - 815-9
AB  - A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI 
      MS/MS) method for the determination of 5 polyether antibiotics (lasalocid, 
      salinomycin, monensin, narasin and maduramicin) in chicken tissues was developed. 
      The polyether antibiotics were extracted from chicken tissues with methanol. The 
      extract was evaporated to dry, and redissolved in hexane, then cleaned up on a 
      Sep-Pak Silica solid-phase extraction cartridge. The target drugs were eluted 
      with 6 mL methylene chloride-methanol (90:10, v/v), and the eluate was collected 
      and dried under a gentle stream of nitrogen gas, then the residue was dissolved 
      with 1 mL acetonitrile (containing 0.1% formic acid) and analyzed by LC-MS/MS. 
      The LC separation was performed on a Symmetry Shield reversed phase C18 bonded 
      silica column with acetonitrile (containing 0.1% formic acid)-0.1% formic acid 
      (97:3, v/v) as mobile phase. The quantification was carried out by positive 
      electrospray ionization and multiple reaction monitoring (MRM) mode. The 
      validation was carried out on spiked chicken muscle (spiked at 0.1 -1500 
      microg/kg) and chicken liver (spiked at 0.2-4500 microg/kg), the average 
      recoveries of target drugs ranged from 71.6%-99.1% with intra-day relative 
      standard deviations (RSDs) of 3.2%-10.7% and inter-day RSDs of 4.6%-14.7%. The 
      limits of quantification (LOQs) in chicken muscle and liver were 0.1-1.0 kg/kg. 
      The results demonstrated that the sensitivity, accuracy and precision of this 
      method meet the requirements of veterinary drug residue analysis. The method is 
      applicable to detect 5 polyether antibiotics in chicken muscle and liver.
FAU - Liang, Chunlai
AU  - Liang C
AD  - College of Veterinary Medicine, China Agricultural University, National Reference 
      Laboratory for Veterinary Drug Residue, Beijing 100193, China.
FAU - Cheng, Linli
AU  - Cheng L
FAU - Shen, Jianzhong
AU  - Shen J
FAU - Zhang, Yujie
AU  - Zhang Y
FAU - Zhang, Suxia
AU  - Zhang S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Ethers)
RN  - 0 (Veterinary Drugs)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/analysis
MH  - Chickens
MH  - Chromatography, Liquid/*methods
MH  - Drug Residues/*analysis
MH  - Ethers/*analysis
MH  - Meat/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tandem Mass Spectrometry/methods
MH  - Veterinary Drugs/analysis
EDAT- 2010/04/01 06:00
MHDA- 2013/12/18 06:00
CRDT- 2010/04/01 06:00
PHST- 2010/04/01 06:00 [entrez]
PHST- 2010/04/01 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 Nov;27(6):815-9.