PMID- 20352942 OWN - NLM STAT- MEDLINE DCOM- 20131212 LR - 20161018 IS - 1000-8713 (Print) IS - 1000-8713 (Linking) VI - 27 IP - 6 DP - 2009 Nov TI - [Determination of four amino acids in tea by capillary zone electrophoresis and direct ultraviolet detection without derivatization]. PG - 840-4 AB - Abstract: Four important amino acids, arginine (Arg), tryptophane (Trp), phenylalanine (Phe) and tyrosine (Tyr) in tea samples with different fermentation processes were simultaneously determined by capillary zone electrophoresis (CZE) using direct ultraviolet detection (UV) at 190 nm. Under the conditions of 20 kV of separation voltage and 25 degrees C of temperature, Arg, Trp, Phe and Tyr were separated successfully within 8 min in 25 mmol/L sodium borate-boric acid (pH 10.0) with the detection limits of 5.0, 1.0, 0.3 and 0.5 mg/L for Arg, Trp, Phe and Tyr, respectively. The relative standard deviations (RSDs) of migration time (n = 7) were all lower than 2.8% and the RSDs of peak electric current (n = 7) were lower than 4.0%. The method was applied in the determination of Arg, Trp, Phe and Tyr in eleven tea samples. The results were satisfactory. This method can provide beneficial reference to evaluation quality of tea. FAU - Wang, Qingping AU - Wang Q AD - Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China. FAU - Zhang, Lan AU - Zhang L FAU - Chen, Guonan AU - Chen G FAU - Lin, Jin-Ming AU - Lin JM LA - chi PT - English Abstract PT - Journal Article PL - China TA - Se Pu JT - Se pu = Chinese journal of chromatography JID - 9424804 RN - 0 (Amino Acids) RN - 0 (Tea) SB - IM MH - Amino Acids/*analysis MH - Electrophoresis, Capillary/*methods MH - Tea/*chemistry EDAT- 2010/04/01 06:00 MHDA- 2013/12/18 06:00 CRDT- 2010/04/01 06:00 PHST- 2010/04/01 06:00 [entrez] PHST- 2010/04/01 06:00 [pubmed] PHST- 2013/12/18 06:00 [medline] PST - ppublish SO - Se Pu. 2009 Nov;27(6):840-4.