PMID- 20353164
OWN - NLM
STAT- MEDLINE
DCOM- 20100818
LR  - 20100430
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 82
IP  - 9
DP  - 2010 May 1
TI  - SPR Biosensing in crude serum using ultralow fouling binary patterned peptide 
      SAM.
PG  - 3699-706
LID - 10.1021/ac100035s [doi]
AB  - Near-zero fouling monolayers based on binary patterned peptides allow low 
      nanomolar detection of the matrix metalloproteinase-3 (MMP-3) directly in crude 
      bovine serum, without sample pretreatment, secondary antibody detection or signal 
      amplification. The peptide 3-MPA-HHHDD-OH (3-MPA, 3-mercaptopropionic acid) was 
      found optimal compared to other binary patterned peptides based on 
      3-MPA-A(x)-B(y)-OH, where 0 <or= x, y <or= 5, and x + y = 5, and compared to PEG. 
      In this study, amino acid A was His, Asp, Ser, or Leu, and amino acid B was His, 
      Asp, or Ser. Zwitterionic peptides and other peptides exhibited excellent 
      resistance to nonspecific adsorption. Binary patterned peptides were capped with 
      3-MPA on the N-terminus providing a monolayer with the C-terminus carboxylic acid 
      available to subsequently immobilize antibodies. Thereby, an IgG biosensor 
      demonstrated the efficiency of binary patterned peptides in SPR biosensing with a 
      detection limit of 1-10 pM in PBS, similar to other optical or electrochemical 
      techniques. This protocol was applied to establish a calibration curve for MMP-3, 
      an analyte of clinical interest for many pathologies and a potential indicator of 
      cancer. The LOD for MMP-3 was 0.14 nM in PBS, with a linearity of up to 50 nM. 
      With the use of PBS calibration, MMP-3 was quantified at low nanomolar in 
      undiluted bovine serum. The SPR response in serum was statistically the same as 
      in PBS. A sensor exposed to blank serum exhibited negligible nonspecific 
      adsorption. Hence, binary patterned peptides are suitable for biosensing directly 
      in complex biological matrixes.
FAU - Bolduc, Olivier R
AU  - Bolduc OR
AD  - Departement de Chimie, Universite de Montreal, Quebec, Canada, H3C 3J7.
FAU - Pelletier, Joelle N
AU  - Pelletier JN
FAU - Masson, Jean-Francois
AU  - Masson JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Blood Proteins)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Peptides)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Biosensing Techniques
MH  - Blood Proteins/*analysis/chemistry
MH  - Cattle
MH  - Enzymes, Immobilized/chemistry
MH  - Matrix Metalloproteinase 3/*analysis/chemistry
MH  - Molecular Sequence Data
MH  - Molecular Structure
MH  - Peptides/*chemistry
MH  - *Surface Plasmon Resonance
EDAT- 2010/04/01 06:00
MHDA- 2010/08/19 06:00
CRDT- 2010/04/01 06:00
PHST- 2010/04/01 06:00 [entrez]
PHST- 2010/04/01 06:00 [pubmed]
PHST- 2010/08/19 06:00 [medline]
AID - 10.1021/ac100035s [doi]
PST - ppublish
SO  - Anal Chem. 2010 May 1;82(9):3699-706. doi: 10.1021/ac100035s.