PMID- 20354773
OWN - NLM
STAT- MEDLINE
DCOM- 20101008
LR  - 20211020
IS  - 1573-6881 (Electronic)
IS  - 0145-479X (Linking)
VI  - 42
IP  - 3
DP  - 2010 Jun
TI  - A resonance Raman band assignable to the O-O stretching mode in the resting 
      oxidized state of bovine heart cytochrome c oxidase.
PG  - 241-3
LID - 10.1007/s10863-010-9282-y [doi]
AB  - In the resting oxidized state (the fully oxidized "as-isolated" state) of 
      cytochrome c oxidase (CcO) preparation, a resonance Raman band is observed at 755 
      cm(-1) upon 647.1 nm excitation in resonance with an absorption band at 655 nm. 
      Addition of cyanide eliminates the Raman band concomitant with loss of the 
      absorption band at 655 nm. These results strongly suggest that the Raman band at 
      755 cm(-1) originates from the O-O stretching mode of the bridging peroxide 
      (Fe-O(-)-O(-)-Cu) in the O(2) reduction site of the fully oxidized "as-isolated" 
      CcO. Although the peroxide bridged structure has been proposed on the basis of 
      X-ray crystallography and reductive titration experiments, the present 
      vibrational spectroscopic analyses reveal conclusively the chemical nature of the 
      bridging ligand at the O(2) reduction site of the fully oxidized "as-isolated" 
      bovine heart CcO.
FAU - Sakaguchi, Miyuki
AU  - Sakaguchi M
AD  - Picobiology Institute, Department of Life Science, Graduate School of Life 
      Science, University of Hyogo, Koto 3-2-1, Kamigori-cho, Ako-gun, Hyogo, 678-1297, 
      Japan.
FAU - Shinzawa-Itoh, Kyoko
AU  - Shinzawa-Itoh K
FAU - Yoshikawa, Shinya
AU  - Yoshikawa S
FAU - Ogura, Takashi
AU  - Ogura T
LA  - eng
PT  - Journal Article
DEP - 20100401
PL  - United States
TA  - J Bioenerg Biomembr
JT  - Journal of bioenergetics and biomembranes
JID - 7701859
RN  - 0 (Peroxides)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Catalytic Domain
MH  - Cattle
MH  - Crystallography, X-Ray
MH  - Electron Transport Complex IV/*chemistry/metabolism
MH  - Myocardium/enzymology
MH  - Oxidation-Reduction
MH  - Peroxides
MH  - Protein Conformation
MH  - Spectrum Analysis, Raman/*methods
EDAT- 2010/04/01 06:00
MHDA- 2010/10/12 06:00
CRDT- 2010/04/01 06:00
PHST- 2010/01/13 00:00 [received]
PHST- 2010/03/07 00:00 [accepted]
PHST- 2010/04/01 06:00 [entrez]
PHST- 2010/04/01 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
AID - 10.1007/s10863-010-9282-y [doi]
PST - ppublish
SO  - J Bioenerg Biomembr. 2010 Jun;42(3):241-3. doi: 10.1007/s10863-010-9282-y. Epub 
      2010 Apr 1.