PMID- 20355181
OWN - NLM
STAT- MEDLINE
DCOM- 20100628
LR  - 20210623
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
VI  - 1
IP  - 2
DP  - 2009 Feb
TI  - Capillary electrophoresis with electrochemiluminescence detection for 
      simultaneous determination of proline and fleroxacin in human urine.
PG  - 87-92
LID - 10.1002/dta.22 [doi]
AB  - The content of free proline (Pro) in body fluids is a biological parameter for 
      patients with renal insufficiency and chronic uraemia. Fleroxacin (FLX) must be 
      used cautiously because of adverse reactions. Its dosage must be adjusted 
      according to the degree of renal insufficiency. In a clinical setting the 
      simultaneous determination of Pro and FLX in body fluids is necessary for the 
      rational utilization of FLX. A capillary electrophoresis (CE) method based on 
      electrochemiluminescence (ECL) detection with Ru(bpy)(3) (2+) was developed for 
      the simultaneous determination of Pro and FLX in human urine. Parameters related 
      to separation and detection were investigated and optimized. The most favourable 
      resolution and high sensitivity were obtained using a 15 mM phosphate buffer at 
      pH 9.6 with the detection potential at 1.15 V. Under optimized conditions, the 
      standard curves were linear in the range of 0.1-80 microg/mL for Pro and 0.1-100 
      microg/mL for FLX. Detection limits(3sigma) of 0.3 ng/mL for FLX and 0.02 
      microg/mL for Pro were obtained. Relative standard derivations (RSDs) of the ECL 
      intensity and the migration time were 3.2% and 0.9% for 4 microg/mL Pro and 3.7% 
      and 1.2% for 4 microg/mL FLX, respectively. The recoveries were in the range of 
      94.8-99.6% for Pro with RSDs of 2.8%-3.6% and 94.7-97.8% for FLX with RSDs of 
      2.9%-3.7%. The proposed method was successfully applied to determine the amounts 
      of Pro and FLX in human urine.
CI  - Copyright 2009 John Wiley & Sons, Ltd.
FAU - Sun, Hanwen
AU  - Sun H
AD  - College of Chemistry and Environmental Science, Hebei University, Key Laboratory 
      of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
FAU - Li, Liqing
AU  - Li L
FAU - Wu, Yuanyuan
AU  - Wu Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Coordination Complexes)
RN  - 0 (tris(2,2-bipyridine)-ruthenium(II))
RN  - 551W113ZEP (2,2'-Dipyridyl)
RN  - 9DLQ4CIU6V (Proline)
RN  - N804LDH51K (Fleroxacin)
SB  - IM
MH  - 2,2'-Dipyridyl/analogs & derivatives
MH  - Adult
MH  - Anti-Infective Agents/*urine
MH  - Coordination Complexes
MH  - Electrophoresis, Capillary/economics/*methods
MH  - Female
MH  - Fleroxacin/*urine
MH  - Humans
MH  - Limit of Detection
MH  - Luminescent Measurements/methods
MH  - Male
MH  - Middle Aged
MH  - Proline/*urine
MH  - Young Adult
EDAT- 2009/02/01 00:00
MHDA- 2010/06/29 06:00
CRDT- 2010/04/01 06:00
PHST- 2010/04/01 06:00 [entrez]
PHST- 2009/02/01 00:00 [pubmed]
PHST- 2010/06/29 06:00 [medline]
AID - 10.1002/dta.22 [doi]
PST - ppublish
SO  - Drug Test Anal. 2009 Feb;1(2):87-92. doi: 10.1002/dta.22.