PMID- 20356565
OWN - NLM
STAT- MEDLINE
DCOM- 20100811
LR  - 20211020
IS  - 1873-4863 (Electronic)
IS  - 0168-1656 (Print)
IS  - 0168-1656 (Linking)
VI  - 147
IP  - 2
DP  - 2010 May 17
TI  - Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: a 
      way for producing functional protein in prokaryotic microorganism.
PG  - 130-5
LID - 10.1016/j.jbiotec.2010.03.013 [doi]
AB  - Based on the bioinformatics analysis of the gene encoding glycoprotein 5 (GP5) of 
      porcine reproductive and respiratory syndrome virus (PRRSV) isolate HH08, two 
      gene fragments were amplified by polymerase chain reaction (PCR), deleting the 
      signal peptide and transmembrane sequences in GP5 gene. Both gene fragments were 
      designated GP5a and GP5b, respectively. They were ligated with a linker and 
      cloned into prokaryotic expression vector, pET-30a. Expression of the protein of 
      interest was induced by isopropyl beta-d-1-thiogalactopyranoside. The purified 
      protein was used as an immunogen to elicit antibody in rabbit. The 
      immunoreactivity of the protein was determined using ELISA and Western blot. 
      Biologically active GP5 and anti-GP5 antibody inhibited cell infection by PRRSV. 
      Moreover, the antibody produced in this study was capable of detecting the cell 
      infection by PRRSV and distinguishing this virus from other viruses.
CI  - 2010 Elsevier B.V. All rights reserved.
FAU - Ren, Xiaofeng
AU  - Ren X
AD  - College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai 
      Street, Xiangfang District, 150030 Harbin, China. rxfemail@yahoo.com.cn
FAU - Wang, Mingcui
AU  - Wang M
FAU - Yin, Jiechao
AU  - Yin J
FAU - Ren, Yudong
AU  - Ren Y
FAU - Li, Guangxing
AU  - Li G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100329
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (Antibodies, Viral)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Cloning, Molecular/*methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Escherichia coli/genetics
MH  - Immunoblotting
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Protein Sorting Signals
MH  - Protein Structure, Tertiary
MH  - Rabbits
MH  - Recombinant Fusion Proteins/chemistry/*genetics/immunology/*metabolism
MH  - Viral Envelope Proteins/chemistry/*genetics/immunology/*metabolism
PMC - PMC7114092
EDAT- 2010/04/02 06:00
MHDA- 2010/08/12 06:00
PMCR- 2010/03/29
CRDT- 2010/04/02 06:00
PHST- 2009/11/18 00:00 [received]
PHST- 2010/03/15 00:00 [revised]
PHST- 2010/03/22 00:00 [accepted]
PHST- 2010/04/02 06:00 [entrez]
PHST- 2010/04/02 06:00 [pubmed]
PHST- 2010/08/12 06:00 [medline]
PHST- 2010/03/29 00:00 [pmc-release]
AID - S0168-1656(10)00158-6 [pii]
AID - 10.1016/j.jbiotec.2010.03.013 [doi]
PST - ppublish
SO  - J Biotechnol. 2010 May 17;147(2):130-5. doi: 10.1016/j.jbiotec.2010.03.013. Epub 
      2010 Mar 29.